Tessler J, Stewart W C, Kresse J I, Snyder M L
Can J Comp Med. 1977 Jan;41(1):127-9.
Markers for differentiating hog cholera and bovine viral diarrhea viruses were studied using Tween 80, chloroform, trichlorotrifluoroethane and tri (n-butyl) phosphate. Attenuated A and virulent Ames strains of hog cholera virus were employed. Moreover, the NADL PK-15 cell culture adopted strain and low cell culture passaged Purdue strain of bovine viral diarrhea virus were used. These viruses were reacted with 2,500 micrograms/ml of Tween 80 for one hour at 37 degrees C. When attenuated A and virulent Ames strains of hog cholera virus with titers greater than 10(6) and 10(5) plaque forming units respectively, were reacted with Tween 80 the titer of each strains was reduced by approximately 10(4) plaque forming units of virus. When either strain of bovine viral diarrhea virus was reacted with Tween 80, virus was not detected.
使用吐温80、氯仿、三氯三氟乙烷和磷酸三（正丁基）酯研究了区分猪瘟病毒和牛病毒性腹泻病毒的标志物。采用了猪瘟病毒的弱毒A株和强毒埃姆斯株。此外，还使用了牛病毒性腹泻病毒的NADL PK - 15细胞培养适应株和低细胞培养传代的普渡株。这些病毒在37℃下与2500微克/毫升的吐温80反应1小时。当猪瘟病毒的弱毒A株和强毒埃姆斯株的滴度分别大于10⁶和10⁵空斑形成单位时，与吐温80反应后，各株病毒的滴度分别降低了约10⁴空斑形成单位的病毒。当牛病毒性腹泻病毒的任何一株与吐温80反应时，均未检测到病毒。
