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从渥太华地区污水中分离出1型脊髓灰质炎病毒的疑似野生毒株。

Isolation of apparently wild strains of poliovirus type 1 from sewage in the Ottawa area.

作者信息

Sattar S A, Westwood J C

出版信息

Can Med Assoc J. 1977 Jan 8;116(1):25-7.

Abstract

In the first 4 months of 1974, 140 gauze pad samples of sewage collected in the Ottawa area were analysed by the BS-C-1 cell system for the presence of viruses pathogenic for humans. Viruses were isolated from 111 (79%) of the samples. Of the 72 (65%) isolates identified by serology and electron microscopic examination, 56 (78%) were reoviruses and 16 (22%), enteroviruses. The enterovirus isolates included one coxsackievirus B4, one vaccine strain of poliovirus type 3, nine vaccine strains of poliovirus type 1 and five strains of poliovirus type 1 that proved by serodifferentiation and temperature marker tests to be different from vaccine strains. The fact that these strains were present in the community sewage in readily detectable concentrations at a time when immunity against polioviruses is declining in such communities is a cause for concern.

摘要

1974年的前4个月,采用BS - C - 1细胞系统对在渥太华地区采集的140份污水纱布垫样本进行分析,以检测其中是否存在对人类致病的病毒。从111份(79%)样本中分离出了病毒。经血清学和电子显微镜检查鉴定的72份(65%)分离株中,56份(78%)为呼肠孤病毒,16份(22%)为肠道病毒。肠道病毒分离株包括1株柯萨奇病毒B4、1株脊髓灰质炎病毒3型疫苗株、9株脊髓灰质炎病毒1型疫苗株以及5株经血清鉴别和温度标记试验证明与疫苗株不同的脊髓灰质炎病毒1型毒株。在这些社区中脊髓灰质炎病毒免疫力正在下降的时候,这些毒株以易于检测到的浓度存在于社区污水中,这一事实令人担忧。

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