用于定量污水中呼肠孤病毒的细胞系、免疫荧光和蚀斑测定程序的评估。
Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage.
作者信息
Ridinger D N, Spendlove R S, Barnett B B, George D B, Roth J C
出版信息
Appl Environ Microbiol. 1982 Apr;43(4):740-6. doi: 10.1128/aem.43.4.740-746.1982.
Abstract
Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage. Madin-Darby bovine kidney (MDBK), rhesus monkey kidney (LLC-MK2), and human embryonic intestinal (intestinal 407) cells were most sensitive, respectively. In a similar study, MDBK cells were more sensitive than LLC-MK2 and Buffalo green monkey kidney (BGM) cells to sewage-isolated, protamine-precipitated reoviruses which had not been serotyped and had no previous cell contact. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count and plaque assay procedures. The immunofluorescence assay is more sensitive and more rapid than the plaque assay. Reoviruses in excess of 10(4)/liter of raw sewage were detected by the immunofluorescent cell count assay.
摘要
对12种连续传代细胞系进行了检测，以确定它们对从污水中分离出的1型、2型和3型呼肠孤病毒的敏感性。结果发现，马迪-达比牛肾（MDBK）细胞、恒河猴肾（LLC-MK2）细胞和人胚胎肠（肠407）细胞分别最为敏感。在一项类似研究中，对于未经血清分型且此前未接触过细胞的污水分离、鱼精蛋白沉淀的呼肠孤病毒，MDBK细胞比LLC-MK2细胞和布法罗绿猴肾（BGM）细胞更敏感。污水分离、鱼精蛋白沉淀的呼肠孤病毒还与MDBK细胞一起用于免疫荧光细胞计数和蚀斑测定程序的比较评估。免疫荧光测定比蚀斑测定更灵敏、更快速。通过免疫荧光细胞计数测定法可检测出每升原污水中超过10⁴个的呼肠孤病毒。
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