Kiani Hamed, Haghighi Ali, Seyyedtabaei Seyyed Javad, Azargashsb Eznollah, Zebardast Nozhat, Taghipour Niloofar, Rostami Ali, Xiao Lihua
Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iranian Veterinary Organization, Hamadan, Iran.
Iran J Parasitol. 2017 Apr-Jun;12(2):169-176.
species are recognized as important gastrointestinal pathogens. This study was conducted to identify the prevalence, clinical manifestations and genotyping of spp. in patients with gastrointestinal illnesses (GIs) in western Iran.
Overall, 1301 fecal samples were collected from patients with GIs referred to the 12 clinical laboratories in Nahavand County, west of Iran. Modified Ziehl-Neelsen staining method was used to identify the oocysts. DNA was extracted from positive samples and spp. were characterized by Nested PCR and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Data analysis was performed using SPSS ver. 16.
Prevalence of cryptosporidiosis was 1.3% (17/1301). infection was significantly associated with vomiting and nausea (=0.001, OR=0.013; CI 95%=0.004- 0.044), abdominal pain (=0.018, OR=0.073; CI 95%=0.008- 0.633) and diarrhea (=0.001, OR=0.092; CI 95%=0.023- 0.362). Of the 17 isolates typed, 11 belonged to the IId subtype family (subtypes IIdA26G1 and IIdA20G1) and six belonged to the IIa subtype family (subtypes IIaA15G2R1 and IIaA16G3R1). There was no significant difference between sub-type families IIa and IId in occurrence of clinical symptoms (= 0.75).
Improved hygiene and avoidance of contact with animals and contaminated soil should be advocated to reduce the occurrence of infections, especially in children.
隐孢子虫属被认为是重要的胃肠道病原体。本研究旨在确定伊朗西部胃肠道疾病（GI）患者中隐孢子虫属的患病率、临床表现及基因分型。
总共从转诊至伊朗西部纳哈万德县12个临床实验室的胃肠道疾病患者中收集了1301份粪便样本。采用改良齐-尼氏染色法鉴定卵囊。从阳性样本中提取DNA，通过巢式PCR和60 kDa糖蛋白（gp60）基因的序列分析对隐孢子虫属进行鉴定。使用SPSS 16.0版进行数据分析。
隐孢子虫病的患病率为1.3%（17/1301）。隐孢子虫感染与呕吐和恶心显著相关（P=0.001，OR=0.013；95%CI=0.004-0.044）、腹痛（P=0.018，OR=0.073；95%CI=0.008-0.633）和腹泻（P=0.001，OR=0.092；95%CI=0.023-0.362）。在分型的17株分离株中，11株属于隐孢子虫IId亚型家族（IIdA26G1和IIdA20G1亚型），6株属于隐孢子虫IIa亚型家族（IIaA15G2R1和IIaA16G3R1亚型）。IIa和IId亚型家族在临床症状出现方面无显著差异（P=0.75）。
应提倡改善卫生条件，避免接触动物和受污染土壤，以减少隐孢子虫感染的发生，尤其是在儿童中。
