Hayashi Yoshihiro, Toda Katsumi, Saibara Toshiji, Okamoto Sumika, Osanai Makoto, Enzan Hideaki, Lee Gang-Hong
Department of Pathology, School of Medicine, Kochi University, Oko-cho, Nankoku, Kochi, 783-8505, Japan.
Cell Tissue Res. 2008 Nov;334(2):219-26. doi: 10.1007/s00441-008-0683-8. Epub 2008 Oct 15.
Fascin-1 is an actin-bundling protein localized at the core actin bundles within microvillar projections and filopodial extensions in migrating cells. It is expressed at a low level in normal epithelial cells, but at a high level in tumor cells, indicating its importance in the invasion and motility of tumor cells. In addition, fascin-1 is expressed in human and murine embryos, occurring at high levels especially in developing nervous tissues. In this study, we have investigated the expression patterns of fascin-1 immunohistochemically during the early stages of rat hepatogenesis. A high expression of fascin-1 was detected in the liver bud and hepatoblasts at embryonic day (ED) 10.5, ED11.5, and ED12.5. Expression fell by ED13.5 and was not detectable at ED14.5. These observations demonstrate that the expression of fascin-1 is correlated with the migration activity of hepatoblasts during the early stages of liver development in rats.
Fascin-1是一种肌动蛋白捆绑蛋白，定位于微绒毛突起内的核心肌动蛋白束以及迁移细胞中的丝状伪足延伸处。它在正常上皮细胞中低水平表达，但在肿瘤细胞中高水平表达，表明其在肿瘤细胞侵袭和运动中的重要性。此外，Fascin-1在人类和小鼠胚胎中表达，尤其在发育中的神经组织中高水平出现。在本研究中，我们通过免疫组织化学方法研究了大鼠肝发生早期阶段Fascin-1的表达模式。在胚胎第10.5天、11.5天和12.5天的肝芽和成肝细胞中检测到Fascin-1的高表达。到胚胎第13.5天表达下降，在胚胎第14.5天未检测到。这些观察结果表明，Fascin-1的表达与大鼠肝脏发育早期成肝细胞的迁移活性相关。
