Shi Shuai, Zheng Hua-Chuan, Zhang- Zhi-Gang
Department of Pathology, Cangzhou People's Hospital, Cangzhou, Hebei 061000, P.R. China.
Mol Clin Oncol. 2020 Aug;13(2):119-128. doi: 10.3892/mco.2020.2069. Epub 2020 Jun 11.
Fascin (encoded by ) is a globular actin cross-linking protein that is required for the formation of actin-based cell surface processes, which are critical for cell migration and cell-matrix adhesion. In the present study, a systematic meta-analysis and bioinformatics analysis was used to identify clinicopathological or prognostic parameters in patients with colorectal cancer. A total of 17 articles were included in the present study obtained from PubMed, Web of Science, Wanfang data, SinoMed and CNKI databases. Odd ratios (ORs) and the corresponding 95% confidence intervals (CIs) were used to estimate the prognostic significance of Fascin expression in patients with colorectal cancer, and the association between Fascin expression and clinicopathological factors. There was a significant correlation between high Fascin expression and poor overall survival rates in patients with colorectal cancer (OR=0.48; 95% CI, 0.38-0.60; P<0.000001). The meta-analysis showed that the expression of Fascin was significantly higher in colorectal cancer tissue compared with the normal mucosa (OR=0.13; 95% CI, 0.10-0.16; P<0.000001) and adenoma (OR=0.23; 95% CI, 0.15-0.34; P<0.000001). Fascin expression was also associated with depth of invasion (OR=0.31; 95% CI, 0.19-0.50; P<0.000001), lymph node metastasis (OR=3.07; 95% CI, 1.72-5.46; P=0.0001), Dukes stage (OR=0.14; 95% CI, 0.04-0.46; P=0.001), Tumor-Node-Metastasis stage (OR=0.38; 95% CI, 0.21-0.71; P=0.003) and dedifferentiation (OR=0.42; 95% CI, 0.19-0.94; P=0.04). According to the bioinformatics analyses, mRNA expression levels were higher in colorectal cancer and adenoma tissues compared with the normal tissues (P<0.05). According to TCGA, mRNA expression was associated with a less favorable prognosis in patients with colorectal cancer as an independent factor (P<0.05), and positively correlated with depth of invasion, microsatellite instability and low serum carcinoembryonic antigen levels in colorectal cancer. Taken together, the results of the present study suggested that Fascin expression is a potential marker of tumorigenesis, aggressiveness and poor prognosis in patients with colorectal cancer.
Fascin（由 编码）是一种球状肌动蛋白交联蛋白，它是基于肌动蛋白的细胞表面突起形成所必需的，而这些突起对于细胞迁移和细胞-基质粘附至关重要。在本研究中，采用系统的荟萃分析和生物信息学分析来确定结直肠癌患者的临床病理或预后参数。本研究共纳入了从PubMed、Web of Science、万方数据、中国生物医学文献数据库和中国知网数据库中获取的17篇文章。使用比值比（OR）和相应的95%置信区间（CI）来评估Fascin表达在结直肠癌患者中的预后意义，以及Fascin表达与临床病理因素之间的关联。结直肠癌患者中Fascin高表达与总体生存率差之间存在显著相关性（OR=0.48；95%CI，0.38-0.60；P<0.000001）。荟萃分析表明，与正常黏膜相比，结直肠癌组织中Fascin的表达显著更高（OR=0.13；95%CI，0.10-0.16；P<0.000001），与腺瘤相比也是如此（OR=0.23；95%CI，0.15-0.34；P<0.000001）。Fascin表达还与浸润深度（OR=0.31；95%CI，0.19-0.50；P<0.000001）、淋巴结转移（OR=3.07；95%CI，1.72-5.46；P=0.0001）、Dukes分期（OR=0.14；95%CI，0.04-0.46；P=0.001）、肿瘤-淋巴结-转移分期（OR=0.38；95%CI，0.21-0.71；P=0.003）和去分化（OR=0.42；95%CI，0.19-0.94；P=0.04）相关。根据生物信息学分析，与正常组织相比，结直肠癌和腺瘤组织中 mRNA表达水平更高（P<0.05）。根据癌症基因组图谱（TCGA）， mRNA表达作为一个独立因素与结直肠癌患者预后较差相关（P<0.05），并且与结直肠癌的浸润深度、微卫星不稳定性和低血清癌胚抗原水平呈正相关。综上所述，本研究结果表明，Fascin表达是结直肠癌患者肿瘤发生、侵袭性和预后不良的一个潜在标志物。
