Wang Bao-Gui, Lohmann Chris P, Riemann Iris, Schubert Harald, Halbhuber Karl-Juergen, König Karsten
Lasermicroscopy Research Unit, Department of Microscopic Anatomy (Anatomy II), Friedrich Schiller University, Jena, Germany.
J Refract Surg. 2008 Oct;24(8):833-9. doi: 10.3928/1081597X-20081001-11.
To evaluate whether corneal flaps can be generated by the 80 MHz near-infrared, intense nanojoule femtosecond laser based on multiphoton absorption.
A solid-state Ti:Sapphire femtosecond laser system was integrated in an inverted JenLab Femt-O-Cut laser scanning microscope. A diffraction-limited 40x objective was used to induce multiphoton ionization and plasma production. New Zealand albino rabbits and porcine eyes were used. Surgical outcomes were determined using frame and line scans with nanojoule pulses at a wavelength of 800 nm.
Surgical performance was assessed by optical imaging, histology, and electron microscopy. No significant corneal turbidity was observed. Optical imaging and histological examinations detected virtually no perturbation in the surrounding tissue. Corneal flaps and stromal lenticules were generated. Wound repair of the unlifted flaps was observed up to 90 days postoperatively.
Surgical results and follow-up studies confirm that this femtosecond laser at nanojoule pulse energy is able to generate corneal flaps precisely, without causing visible collateral damage to the surrounding tissue or overlying epithelium.
评估基于多光子吸收的80兆赫兹近红外强纳焦耳飞秒激光能否制作角膜瓣。
将一台固态钛宝石飞秒激光系统集成到倒置的JenLab Femt - O - Cut激光扫描显微镜中。使用衍射极限的40倍物镜诱导多光子电离和等离子体产生。使用新西兰白化兔和猪眼。通过使用波长为800纳米的纳焦耳脉冲进行帧扫描和线扫描来确定手术结果。
通过光学成像、组织学和电子显微镜评估手术效果。未观察到明显的角膜混浊。光学成像和组织学检查几乎未检测到周围组织有扰动。制作出了角膜瓣和基质透镜。术后长达90天观察到未掀起的角膜瓣的伤口修复情况。
手术结果和随访研究证实，这种纳焦耳脉冲能量的飞秒激光能够精确制作角膜瓣，而不会对周围组织或上方的上皮造成可见的附带损伤。
