Wang Bao-Gui, Riemann Iris, Schubert Harald, Halbhuber Karl-Juergen, Koenig Karsten
Institute of Microscopic Anatomy (Anatomy II), University of Jena, Teichgraben 7, 07743, Jena, Germany.
Cell Tissue Res. 2007 Jun;328(3):515-20. doi: 10.1007/s00441-006-0367-1. Epub 2007 Feb 14.
Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery for the treatment of visual disorders.
通过使用80兆赫兹近红外强纳焦耳飞秒激光脉冲，在活体兔角膜中进行了非侵入性组织内消融。组织内手术效果是由800纳米波长的多光子吸收诱导产生的，并通过组织学检查得以确定。获得了高度精确的组织内消融，且对上层或下层均无有害影响。术后通过双光子成像检测激光治疗角膜中活化的角膜细胞。因此，组织内飞秒激光消融作为一种治疗视力障碍的屈光手术有效技术具有潜力。
