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Automated aflatoxin analysis of foods and animal feeds using immunoaffinity column clean-up and high-performance liquid chromatographic determination.

作者信息

Sharman M, Gilbert J

机构信息

Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Norwich, U.K.

出版信息

J Chromatogr. 1991 Apr 26;543(1):220-5. doi: 10.1016/s0021-9673(01)95771-1.

DOI:10.1016/s0021-9673(01)95771-1
PMID:1885682
Abstract

A commercially available system is described for the fully automated clean-up and high-performance liquid chromatographic (HPLC) analysis of aflatoxins in foods and animal feeds. The system marketed primarily for handling solid-phase extraction columns has modified software to facilitate use with immunoaffinity columns. Sample extract clean-up followed by injection onto an HPLC column with post-column iodination and fluorescence detection is carried out completely unattended. A coefficient of variation of 5.1% for aflatoxin B1 analysis was obtained, and the accuracy of the system was demonstrated by the analysis of peanut butter certified reference material.

摘要

相似文献

1
Automated aflatoxin analysis of foods and animal feeds using immunoaffinity column clean-up and high-performance liquid chromatographic determination.
J Chromatogr. 1991 Apr 26;543(1):220-5. doi: 10.1016/s0021-9673(01)95771-1.
2
Determination of aflatoxin B1 levels in peanut butter using an immunoaffinity column clean-up procedure: inter-laboratory study.采用免疫亲和柱净化程序测定花生酱中黄曲霉毒素B1的含量：实验室间研究
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The preparation, validation and certification of the aflatoxin content of two peanut butter reference materials.两种花生酱标准物质黄曲霉毒素含量的制备、验证及认证
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[Studies on chemical analysis of mycotoxin (XXI). A rapid analytical method for aflatoxins by immunoaffinity column chromatography and high performance liquid chromatography].[霉菌毒素的化学分析研究（XXI）。免疫亲和柱色谱法和高效液相色谱法快速分析黄曲霉毒素]
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Simultaneous determination of aflatoxins B₁, B₂, G₁, and G₂ in foods and feed materials.食品和饲料原料中黄曲霉毒素B₁、B₂、G₁和G₂的同时测定。
Methods Mol Biol. 2011;739:203-10. doi: 10.1007/978-1-61779-102-4_18.

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A Review: Sample Preparation and Chromatographic Technologies for Detection of Aflatoxins in Foods.综述：食品中黄曲霉毒素检测的样品制备及色谱技术
Toxins (Basel). 2020 Aug 21;12(9):539. doi: 10.3390/toxins12090539.
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Analytical methods for the determination of aflatoxins in various food matrices at concentrations regarding the limits set in european regulations: development, characteristics, limits.
各种食品基质中黄曲霉毒素浓度的分析方法：发展、特点、限量。
Mycotoxin Res. 2000 Mar;16(1):23-42. doi: 10.1007/BF02946103.
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Formation of ochratoxin A and aflatoxins following the use of propionic acid as a grain preservative for storing damp barley.丙酸作为谷物防腐剂储存受潮大麦后赭曲霉毒素 A 和黄曲霉毒素的形成。
Mycotoxin Res. 2004 Jun;20(2):68-79. doi: 10.1007/BF02946737.