Niedwetzki G, Lach G, Geschwill K
Dr. Wiertz-Dipl.-Chem. Eggert-Dr. Jörissen GmbH, Handels- und Umweltschutzlaboratorium, Hamburg, Germany.
J Chromatogr A. 1994 Feb 11;661(1-2):175-80. doi: 10.1016/0021-9673(94)85188-3.
An automated HPLC method with postcolumn derivatization is described for the determination of aflatoxins B1, B2, G1 and G2 after an immunoaffinity column clean-up. It can be used for the determination of aflatoxins in a variety of foodstuffs such as nuts, nut-like products (pistachios, almonds, etc.) and dried fruit. The aflatoxins are extracted with methanol-water, followed by a filtration step. Dilution of the extract, mixing, immunoaffinity column clean-up, elution of the aflatoxins and optional on-line HPLC are performed by an automatic work station (Zymark BenchMate). The subsequent HPLC analysis includes a postcolumn derivatization step with iodine solution and fluorimetric detection. The method compared well with manual techniques and another automated method.
本文描述了一种采用柱后衍生化的自动高效液相色谱法，用于在免疫亲和柱净化后测定黄曲霉毒素B1、B2、G1和G2。它可用于测定各种食品中的黄曲霉毒素，如坚果、类似坚果的产品（开心果、杏仁等）和干果。黄曲霉毒素用甲醇 - 水提取，随后进行过滤步骤。提取物的稀释、混合、免疫亲和柱净化、黄曲霉毒素的洗脱以及可选的在线高效液相色谱分析均由自动工作站（Zymark BenchMate）完成。后续的高效液相色谱分析包括用碘溶液进行柱后衍生化步骤和荧光检测。该方法与手动技术和另一种自动方法相比效果良好。
