Corbett A D, Gillan M G, Kosterlitz H W
Department of Physiology, University of Aberdeen Marischal College, UK.
J Recept Res. 1991;11(1-4):665-73. doi: 10.3109/10799899109066434.
Preparations of guinea-pig myenteric plexus-longitudinal muscle suspended in Krebs solution were stimulated electrically in the presence of cycloheximide and tetraethylammonium. The amounts of eleven endogenous opioid peptides released into the perifusing Krebs solution were determined and correlated with the decrease in the tissue contents induced by stimulation. For pro-enkephalin fragments the ratio of release to reduction in tissue contents was 29 to 43% for [Met]enkephalin, [Leu]enkephalin, [Met]enkephalyl-RF and [Met]enkephalyl-RGL. With [Met]enkephalyl-RRV-NH2 (BAM-8) the ratio was higher by 50% or more. However, it is of interest that there was no release of the probable precursor [Met]enkephalyl-RRVGRPEWWMDYQ(BAM-18). In this context it may be important that BAM-8 is the only endogenous opioid peptide having -NH2 at the C-terminal. The low tissue levels of pro-dynorphin derived peptide have made estimation of release unreliable.
将豚鼠肠肌间神经丛 - 纵行肌标本置于克雷布斯溶液中，在环己酰亚胺和四乙铵存在的情况下进行电刺激。测定释放到灌注用克雷布斯溶液中的11种内源性阿片肽的量，并将其与刺激引起的组织含量减少相关联。对于前脑啡肽片段，[甲硫氨酸]脑啡肽、[亮氨酸]脑啡肽、[甲硫氨酸]脑啡肽 - RF和[甲硫氨酸]脑啡肽 - RGL的释放量与组织含量减少量的比值为29%至43%。对于[甲硫氨酸]脑啡肽 - RRV - NH2（BAM - 8），该比值高出50%或更多。然而，有趣的是，可能的前体[甲硫氨酸]脑啡肽 - RRVGRPEWWMDYQ（BAM - 18）并未释放。在这种情况下，BAM - 8是唯一在C末端具有 - NH2的内源性阿片肽这一点可能很重要。前强啡肽衍生肽的组织水平较低，使得释放量的估计不可靠。
