Joffret M L, Zanetti C, Morgeaux S, Leclerc C, Sureau P, Perrin P
Unité de la Rage, Institut Pasteur, Paris, France.
Biologicals. 1991 Apr;19(2):113-23. doi: 10.1016/1045-1056(91)90009-9.
The potency of different rabies vaccines was measured via cell mediated immunity (CMI) assessed by the production of interleukin-2 (IL-2) by CD4+CD8- lymphocytes. IL-2 production by splenocytes from mice immunized with various vaccines was measured following in vitro stimulation with antigens from different rabies and rabies-related strains. IL-2 production was specific, reproducible and correlated with the vaccine protective activity as determined by the pre-exposure NIH test. Our results suggest that measurement of IL-2 production could be used for the appraisal of rabies vaccine potency.
