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新生牛腹泻病毒的空斑试验及人血清中的中和抗体

Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera.

作者信息

Matsuno S, Inouye S, Kono R

出版信息

J Clin Microbiol. 1977 Jan;5(1):1-4. doi: 10.1128/jcm.5.1.1-4.1977.

DOI:10.1128/jcm.5.1.1-4.1977
PMID:188863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274520/
Abstract

Neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of MA-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. By using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. It was demonstrated that some human sera contained neutralizing antibody to this agent.

摘要

新生牛腹泻病毒（一种牛轮状病毒），当在覆盖培养基中加入二乙氨基乙基葡聚糖和胰蛋白酶时，能在恒河猴肾细胞系MA - 104细胞单层上形成明显的蚀斑。通过使用这种蚀斑测定法，对新生牛腹泻病毒中和抗体的滴定成为可能。结果表明，一些人血清中含有针对该病原体的中和抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d6b/274520/f221b2de3911/jcm00210-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d6b/274520/f221b2de3911/jcm00210-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d6b/274520/f221b2de3911/jcm00210-0023-a.jpg

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本文引用的文献

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Cell culture propagation of neonatal calf diarrhea (scours) virus.新生犊牛腹泻病毒的细胞培养增殖
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Passive immunity to bovine rotavirus in newborn calves fed colostrum supplements from immunized or nonimmunized cows.给新生小牛喂食来自免疫或未免疫母牛的初乳补充剂后，小牛对牛轮状病毒的被动免疫。
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Science. 1974 Sep 20;185(4156):1049-53. doi: 10.1126/science.185.4156.1049.
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Cell culture studies of a neonatal calf diarrhea virus.新生小牛腹泻病毒的细胞培养研究
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Letter: Virus particles in gastroenteritis.信函：肠胃炎中的病毒颗粒。
Lancet. 1973 Dec 29;2(7844):1497. doi: 10.1016/s0140-6736(73)92760-8.
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Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis.急性非细菌性胃肠炎患儿十二指肠黏膜上皮细胞中的病毒颗粒。
Lancet. 1973 Dec 8;2(7841):1281-3. doi: 10.1016/s0140-6736(73)92867-5.
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Letter: Duovirus in schoolchildren with gastroenteritis.
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New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen.针对婴儿肠胃炎人呼肠孤病毒样病原体的新型补体结合试验。以内布拉斯加小牛腹泻病毒作为抗原。
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