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使用单克隆抗体识别常规处理的骨活检标本中的破骨细胞。

Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens.

作者信息

Athanasou N A, Puddle B, Quinn J, Woods C G

机构信息

Nuffield Department of Pathology, University of Oxford, John Radcliffe Hospital, Headington.

出版信息

J Clin Pathol. 1991 Aug;44(8):664-6. doi: 10.1136/jcp.44.8.664.

DOI:10.1136/jcp.44.8.664
PMID:1890200
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC496760/
Abstract

In decalcified (5% nitric acid) and undecalcified (glycol-methacrylate or resin embedded) routinely processed bone specimens osteoclasts against resorbing surfaces were identified with monoclonal antibodies directed against leucocyte common antigen (LCA) (PD7/26, 2B11), CD68 (KP1), and gpIIIa (Y2/51) but not against HLA-DR (CR3/43 and Ta11B5). Mononuclear cells on resorbing surfaces and occasional mononuclear cells against or near resting surfaces showed a similar pattern of reactivity. This study shows that immunohistochemistry is a sensitive and useful technique for identifying osteoclasts in routinely processed bone specimens. It also suggests a role for mononuclear cells (possibly pre-osteoclasts) in bone resorption.

摘要

在脱钙（5%硝酸）和未脱钙（乙二醇甲基丙烯酸酯或树脂包埋）的常规处理骨标本中，用针对白细胞共同抗原（LCA）（PD7/26、2B11）、CD68（KP1）和gpIIIa（Y2/51）的单克隆抗体鉴定了与吸收表面相对的破骨细胞，但未鉴定出与HLA-DR（CR3/43和Ta11B5）相对的破骨细胞。吸收表面的单核细胞以及偶尔与静止表面相对或靠近静止表面的单核细胞显示出相似的反应模式。本研究表明，免疫组织化学是在常规处理骨标本中鉴定破骨细胞的一种敏感且有用的技术。它还提示单核细胞（可能是破骨细胞前体细胞）在骨吸收中起作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/e2ccd22a8195/jclinpath00410-0049-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/b96f575eb769/jclinpath00410-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/51040f569004/jclinpath00410-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/e2ccd22a8195/jclinpath00410-0049-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/b96f575eb769/jclinpath00410-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/51040f569004/jclinpath00410-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d74/496760/e2ccd22a8195/jclinpath00410-0049-c.jpg

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Immunophenotype of multinucleated and mononuclear cells in giant cell lesions of bone and soft tissue.骨与软组织巨细胞病变中多核及单核细胞的免疫表型
J Clin Pathol. 1992 May;45(5):398-404. doi: 10.1136/jcp.45.5.398.
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When is it an osteoclast?它何时是破骨细胞？
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Diagnosis of human lymphoma with monoclonal antileukocyte antibodies.用单克隆抗白细胞抗体诊断人类淋巴瘤。
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Use of monoclonal antibodies for the histopathological diagnosis of human malignancy.单克隆抗体在人类恶性肿瘤组织病理学诊断中的应用。
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Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens.针对骨巨细胞瘤（巨细胞骨肿瘤）的单克隆抗体：破骨细胞特异性细胞抗原的定义
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