van den Hoek J A, Al E J, Huisman J G, Goudsmit J, Coutinho R A
Department of Public Health and Environment, Municipal Health Service of Amsterdam, The Netherlands.
J Med Virol. 1991 Jun;34(2):100-3. doi: 10.1002/jmv.1890340206.
The prevalence of human T-cell leukaemia virus-I and -II infection was studied in a cohort of 346 intravenous and nonintravenous drug users in Amsterdam. Three participants (0.86%) had antibodies to HTLV-I by two commercially available HTLV-I enzyme immunoassays (EIA). Infection in these three subjects was confirmed by radioimmunoprecipitation assay. In the immunoblot study, only two of the three subjects were considered positive, since the serum of the third subject had antibodies to p24 only. By means of the polymerase chain reaction two participants (male intravenous drug users infected with human immunodeficiency virus; HIV) appeared to be infected with HTLV-I and one subject (a male nonintravenous drug user from Surinam) with HTLV-II. It is concluded that HTLV-I and HTLV-II circulate sporadically among drug users in Amsterdam and that risky injecting behaviour, which led to an HIV epidemic among intravenous drug users, has not led so far to an appreciable transmission of the other retroviruses among this group.
在阿姆斯特丹的一组346名静脉注射吸毒者和非静脉注射吸毒者中，对人T细胞白血病病毒-I和-II感染的流行情况进行了研究。通过两种市售的HTLV-I酶免疫测定法（EIA），有三名参与者（0.86%）检测出抗HTLV-I抗体。这三名受试者的感染通过放射免疫沉淀试验得到证实。在免疫印迹研究中，三名受试者中只有两名被认为呈阳性，因为第三名受试者的血清仅含有抗p24抗体。通过聚合酶链反应，两名参与者（感染了人类免疫缺陷病毒；HIV的男性静脉注射吸毒者）似乎感染了HTLV-I，一名受试者（来自苏里南的男性非静脉注射吸毒者）感染了HTLV-II。得出的结论是，HTLV-I和HTLV-II在阿姆斯特丹的吸毒者中呈散发性传播，导致静脉注射吸毒者中出现HIV流行的危险注射行为，到目前为止尚未导致该群体中其他逆转录病毒的明显传播。
