病毒膜的组装。I. 水泡性口炎病毒膜蛋白与无细胞体系中膜的结合
Assembly of viral membranes. I. Association of vesicular stomatitis virus membrane proteins and membranes in a cell-free system.
作者信息
Morrison T G, McQuain C
出版信息
J Virol. 1977 Feb;21(2):451-8. doi: 10.1128/JVI.21.2.451-458.1977.
Abstract
We report here an in vitro system designed to study the interactions of vesicular stomatitis virus (VSV) proteins with cellular membranes. We have synthesized the VSV nucleocapsid (N) protein, nonstructural (NS) protein, glycoprotein (G protein), and membrane (M) protein in a wheat germ, cell-free, protein-synthesizing system directed by VSV 12 to 18S RNA. When incubated at low salt concentrations with purified cytoplasmic membranes derived from Chinese hamster ovary cells, the VSV M andG proteins bind to membranes, whereas the VSV N and NS proteins do not. The VSV M protein binds to membranes in low or high divalent cation concentrations, whereas binding of significant amounts of G protein requires at least 5 mM magnesium acetate concentrations.
摘要
我们在此报告一种体外系统，其设计用于研究水泡性口炎病毒（VSV）蛋白与细胞膜的相互作用。我们已在由VSV 12至18S RNA指导的麦胚无细胞蛋白质合成系统中合成了VSV核衣壳（N）蛋白、非结构（NS）蛋白、糖蛋白（G蛋白）和膜（M）蛋白。当在低盐浓度下与源自中国仓鼠卵巢细胞的纯化细胞质膜一起孵育时，VSV M蛋白和G蛋白会与膜结合，而VSV N蛋白和NS蛋白则不会。VSV M蛋白在低或高浓度二价阳离子条件下均可与膜结合，而大量G蛋白的结合至少需要5 mM乙酸镁浓度。
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