McGregor S, Rueckert R R
J Virol. 1977 Feb;21(2):548-53. doi: 10.1128/JVI.21.2.548-553.1977.
Cytoplasmic extracts of rhinovirus 1A-infected HeLa cells, pulsed 15 min with [3H]leucine, contained a 13S subunit which was rich in the capsid precursor, peptide 92. After a 30-min chase, most of the capsid-related protein sedimented in a 14S peak that contained equimolar amounts of the capsid peptides epsilon, alpha, and gamma, and some residual chain 92. The 14S subunit could be dissociated at pH 4.8 into 6S subunits containing only epsilon, alpha, and gamma chains in equal proportions, indicating that the 14S subunit is an oligomer of (epsilon gamma alpha) protomers. These subunits resemble subunits previously identified in the assembly of enteroviruses. These observations support the idea that rhinovirus assembly is basically similar to that of enteroviruses. Comparative studies on the peptide stoichiometry of the virion and the capsid precursor subunits indicate that rhinovirus 1A can contain as many as 11 immature protomers per virion.
用[³H]亮氨酸脉冲标记15分钟的人鼻病毒1A感染的HeLa细胞的细胞质提取物，含有一个富含衣壳前体肽92的13S亚基。经过30分钟的追踪，大多数衣壳相关蛋白沉淀在一个14S峰中，该峰含有等摩尔量的衣壳肽ε、α和γ，以及一些残留的92链。14S亚基在pH 4.8时可解离成仅含有等量ε、α和γ链的6S亚基，这表明14S亚基是(εγα)原体的寡聚体。这些亚基类似于先前在肠道病毒组装中鉴定出的亚基。这些观察结果支持鼻病毒组装与肠道病毒基本相似的观点。对病毒体和衣壳前体亚基的肽化学计量学的比较研究表明，每个病毒体的人鼻病毒1A可能含有多达11个未成熟的原体。
