通过1H NMR对由d(GGTTTTTGG)形成的四分子复合物中糖苷键构象的表征。
Characterization by 1H NMR of glycosidic conformations in the tetramolecular complex formed by d(GGTTTTTGG).
作者信息
Wang Y, Jin R, Gaffney B, Jones R A, Breslauer K J
机构信息
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
出版信息
Nucleic Acids Res. 1991 Sep 11;19(17):4619-22. doi: 10.1093/nar/19.17.4619.
Abstract
We have conducted two dimensional NOESY studies on the molecule d(G2T5G2) to characterize the structure of the tetramolecular complex previously identified by calorimetric and spectroscopic studies (1). Analysis of the NOE and exchange cross peaks observed in the NOESY spectra establishes the formation of structured conformations at low temperature (5 degrees C). Significantly, within each strand of these structured conformations, the G1 and G8 residues adopt syn glycosidic torsion angles, while the G2 and G9 residues adopt anti glycosidic torsion angles. Consequently, any structure proposed for the tetramolecular complex of d(G2T5G2) must have alternating G(syn) and G(anti) glycosidic torsion angles within each strand. The implications of this observation for potential structures of the tetramolecular complex of d(G2T5G2) are discussed.
摘要
我们对d(G2T5G2)分子进行了二维NOESY研究，以表征先前通过量热法和光谱研究确定的四分子复合物的结构(1)。对在NOESY光谱中观察到的NOE和交换交叉峰的分析确定了在低温(5摄氏度)下形成了结构化构象。值得注意的是，在这些结构化构象的每一条链内，G1和G8残基采用顺式糖苷扭转角，而G2和G9残基采用反式糖苷扭转角。因此，为d(G2T5G2)的四分子复合物提出的任何结构在每条链内都必须具有交替的G(顺式)和G(反式)糖苷扭转角。本文讨论了这一观察结果对d(G2T5G2)四分子复合物潜在结构的影响。
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