Schulenburg B J, Plapp F V, Rachel J M
Community Blood Center of Greater Kansas City, Missouri.
Transfusion. 1991 Sep;31(7):633-5. doi: 10.1046/j.1537-2995.1991.31791368341.x.
A solid-phase red cell adherence (SPRCA) assay has been developed to screen blood donors for IgA deficiency, and 6117 donor sera have been screened by this method. Eighteen sera were found to be IgA deficient, which represents a frequency of 1 in 340. Seventeen of these sera were retested by passive hemagglutination inhibition, which has a sensitivity of approximately 0.1 mg per dL. Eight sera were confirmed as IgA deficient, and nine were found to contain low levels of IgA (less than 1 mg/dL). The approximate sensitivity of the SPRCA assay is 1 mg per dL. The speed, simplicity, and sensitivity of this assay make it a good alternative to conventional methods of screening.
已开发出一种固相红细胞黏附（SPRCA）检测方法用于筛查献血者的IgA缺乏症，并用该方法筛查了6117份献血者血清。发现18份血清IgA缺乏，频率为1/340。其中17份血清通过被动血凝抑制法重新检测，该方法的灵敏度约为每分升0.1毫克。8份血清被确认为IgA缺乏，9份血清被发现含有低水平的IgA（低于1毫克/分升）。SPRCA检测方法的近似灵敏度为每分升1毫克。该检测方法的速度、简便性和灵敏度使其成为传统筛查方法的良好替代方法。
