来自复杂双链DNA病毒的重复序列可用作DNA指纹识别的小卫星探针。

Repeat sequences from complex ds DNA viruses can be used as minisatellite probes for DNA fingerprinting.

作者信息

Crawford A M, Buchanan F C, Fraser K M, Robinson A J, Hill D F

机构信息

Department of Biochemistry, University of Otago, Dunedin, New Zealand.

出版信息

Anim Genet. 1991;22(2):177-81. doi: 10.1111/j.1365-2052.1991.tb00660.x.

DOI:10.1111/j.1365-2052.1991.tb00660.x

PMID:1892249

Abstract

In a search for new fingerprinting probes for use with sheep, repeat sequences derived from five poxviruses, an iridovirus and a baculovirus were screened against DNA from sheep pedigrees. Probes constructed from portions of the parapox viruses, orf virus and papular stomatitis virus and the baculovirus from the alfalfa looper, Autographa californica, nuclear polyhedrosis virus all gave fingerprint patterns. Probes from three other poxviruses and an iridovirus did not give useful banding patterns.

摘要

为寻找用于绵羊的新型指纹识别探针，针对来自绵羊家系的DNA，筛选了源自五种痘病毒、一种虹彩病毒和一种杆状病毒的重复序列。由副痘病毒、口疮病毒和丘疹性口炎病毒的部分序列以及苜蓿银纹夜蛾核型多角体病毒（Autographa californica nuclear polyhedrosis virus）构建的探针均产生了指纹图谱。来自其他三种痘病毒和一种虹彩病毒的探针未产生有用的条带模式。