用中性红细胞活力测定法测定T-2毒素及其代谢产物的细胞毒性。
Cytotoxicity of T-2 toxin and its metabolites determined with the neutral red cell viability assay.
作者信息
Babich H, Borenfreund E
机构信息
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
出版信息
Appl Environ Microbiol. 1991 Jul;57(7):2101-3. doi: 10.1128/aem.57.7.2101-2103.1991.
Abstract
The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2, the sequence of potency for a series of mycotoxins was T-2 greater than HT-2 greater than T-2 triol greater than T-2 tetraol.
摘要
采用中性红（NR）细胞活力测定法，对多种人类来源的细胞类型进行检测，以定量分析T-2毒素及其代谢产物的效力。人类黑色素瘤SK-Mel/27细胞系最为敏感，其半数细胞毒性值为每毫升2.8纳克T-2毒素。对于人类肝癌细胞系HepG2，一系列霉菌毒素的效力顺序为：T-2毒素＞HT-2毒素＞T-2三醇＞T-2四醇。
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