Abbas H K, Shier W T, Mirocha C J
J Assoc Off Anal Chem. 1984 May-Jun;67(3):607-10.
Cultured human diploid fibroblasts and a cloned permanent line of Swiss mouse fibroblasts (3T3) have been adapted to a biological assay of trichothecenes and other toxic fungal metabolites both in purified form and as crude extracts. The assays are carried out in multi-well tissue culture trays with serial dilutions of toxins in 200 microL volumes of medium. Cells are cultured with the toxins for 3-4 days, fixed, and stained, providing a permanent record of results. The sensitivity of the assay ranges from 0.1 ng/mL (20 pg/assay) for T-2 toxin on mouse fibroblasts to no detectable toxicity at 5 micrograms/mL for cis-zearalenone and verrucarol. Human skin fibroblasts exhibit patterns of relative sensitivities similar to that observed with mouse fibroblasts, but the absolute sensitivities are much greater with mouse fibroblasts because they can be cultured at much lower cell densities.
培养的人二倍体成纤维细胞和瑞士小鼠成纤维细胞的克隆永久系（3T3）已适用于对单端孢霉烯族毒素和其他有毒真菌代谢产物进行生物测定，这些代谢产物既有纯化形式，也有粗提物形式。测定在多孔组织培养板中进行，毒素在200微升培养基中进行系列稀释。细胞与毒素一起培养3 - 4天，固定并染色，从而提供结果的永久记录。该测定的灵敏度范围从对小鼠成纤维细胞的T - 2毒素的0.1纳克/毫升（每次测定20皮克）到顺式玉米赤霉烯酮和疣孢菌素在5微克/毫升时无明显毒性。人皮肤成纤维细胞表现出与小鼠成纤维细胞相似的相对敏感性模式，但小鼠成纤维细胞的绝对敏感性要高得多，因为它们可以在低得多的细胞密度下培养。
