Kalmykova E N, Dergunova E S, Ermolaeva T N, Gorshkova R P, Komandrova N A
Prikl Biokhim Mikrobiol. 2008 Jul-Aug;44(4):410-6.
The utility of a piezoquartz immunosensor coated with lipopolysaccharides (LPS) for the quantification of antibody specificities was demonstrated. Immunochemical reactions were monitored according to the changes in the weight of sensor bioreceptor layer with high sensitivity (detection limit, 1.3 microg/ml) and assay rate (10 min) without any additional labels. The capabilities of this sensor were demonstrated by the example of quantifying the cross-reactivity of blood serum antibodies with the LPS of Yersinia enterocolitica serotypes O:3, O:5, O:5.27, O:6.30, and O:6.31. The proposed approach is promising for clinical diagnostics of yersiniosis, an infectious intestinal disease.
已证明涂有脂多糖（LPS）的压电石英免疫传感器在定量抗体特异性方面的实用性。通过高灵敏度（检测限为1.3微克/毫升）和测定速率（10分钟）监测传感器生物受体层重量的变化来监测免疫化学反应，无需任何额外标记。通过定量血清抗体与小肠结肠炎耶尔森菌血清型O:3、O:5、O:5.27、O:6.30和O:6.31的LPS的交叉反应性实例，证明了该传感器的功能。所提出的方法在耶尔森菌病（一种传染性肠道疾病）的临床诊断方面很有前景。
