Monti L D, Sandoli P E, Costa S, Phan V C, Piatti P M
Laboratorio Metabolismo Intermedio L15 Istituto Scientifico San Raffaele University of Milano Via Olgettina 60 Milano 20132 Italy.
J Automat Chem. 1993;15(5):177-81. doi: 10.1155/S1463924693000240.
Intermediate products of the metabolism of glucose, fat and amino-acid are important in the evaluation of such metabolic disorders as diabetes mellitus, liver disease and metabolic acidosis. In the present study, methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, beta-hydroxybutyrate and glycerol) have been adapted to a fast centrifugal analyzer: the COBAS FARA. Correlation coeffcients rangedfrom 0.90 to 0.99, compared to established manual spectrophotometric methods. Within-run coeffcients of variation (CVs) ranged between 2.9 and 8.8% at low levels, between 1.5 and 5.7% at medium levels and between 1.2 and 5.6% at high levels. Between-run CVs were between 4.0 and 15.0% at low levels, between 1.7 and 7.0% at medium levels and between 1.3 and 2.7% at high levels. These fluorimetric assays for the determination of intermediate metabolites on COBAS FARA (Roche) have a good sensitivity and precision, are less costly than manual methods and can be used on a routine basis.
葡萄糖、脂肪和氨基酸代谢的中间产物在评估诸如糖尿病、肝病和代谢性酸中毒等代谢紊乱方面具有重要意义。在本研究中，用于测量中间代谢产物（乳酸、丙酮酸、丙氨酸、β-羟基丁酸和甘油）的方法已应用于一种快速离心分析仪：COBAS FARA。与既定的手动分光光度法相比，相关系数在0.90至0.99之间。批内变异系数（CVs）在低水平时为2.9%至8.8%，中等水平时为1.5%至5.7%，高水平时为1.2%至5.6%。批间CVs在低水平时为4.0%至15.0%，中等水平时为1.7%至7.0%，高水平时为1.3%至2.7%。这些用于在COBAS FARA（罗氏）上测定中间代谢产物的荧光测定法具有良好的灵敏度和精密度，比手动方法成本更低，可常规使用。
