Harrison J, Hodson A W, Skillen A W, Stappenbeck R, Agius L, Alberti K G
Department of Clinical Biochemistry and Metabolic Medicine, Medical School, Newcastle upon Tyne, UK.
J Clin Chem Clin Biochem. 1988 Mar;26(3):141-6. doi: 10.1515/cclm.1988.26.3.141.
Methods are described for the analysis of glucose, lactate, pyruvate, alanine, glycerol, 3-hydroxybutyrate and acetoacetate in perchloric acid extracts of human blood, using the Cobas Bio centrifugal analyser fitted with a fluorimetric attachment. Intra-assay and inter-assay coefficients of variation ranged from 1.9 to 7.9% and from 1.0 to 7.2% respectively. Correlation coefficients ranged from 0.96 to 0.99 against established continuous-flow and manual spectrophotometric methods. All seven metabolites can be measured using a single perchloric acid extract of 20 microliter of blood. The versatility of the assays is such that as little as 100 pmol pyruvate, 3-hydroxybutyrate or as much as 15 nmol glucose can be measured in the same 20 microliter extract.
介绍了使用配备荧光附件的Cobas Bio离心分析仪分析人血高氯酸提取物中葡萄糖、乳酸、丙酮酸、丙氨酸、甘油、3-羟基丁酸和乙酰乙酸的方法。批内和批间变异系数分别为1.9%至7.9%和1.0%至7.2%。与既定的连续流动和手动分光光度法相比，相关系数在0.96至0.99之间。所有七种代谢物都可以使用20微升血液的单一高氯酸提取物进行测量。这些测定方法的通用性在于，在同一20微升提取物中可以测量低至100皮摩尔的丙酮酸、3-羟基丁酸或高达15纳摩尔的葡萄糖。
