Adams Lynn S, Phung Sheryl, Wu Xiwei, Ki Lui, Chen Shiuan
Department of Surgical Research, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
Nutr Cancer. 2008;60(6):744-56. doi: 10.1080/01635580802192866.
White button mushrooms are a widely consumed food containing phytochemicals beneficial to cancer prevention. The purpose of this research was to evaluate the effects of white button mushroom extract and its major component, conjugated linoleic acid (CLA) on prostate cancer cell lines in vitro and mushroom extract in vivo. In all cell lines tested, mushroom inhibited cell proliferation in a dose-dependent manner and induced apoptosis within 72 h of treatment. CLA inhibited proliferation in the prostate cancer cell lines in vitro. DU145 and PC3 prostate tumor size and tumor cell proliferation were decreased in nude mice treated with mushroom extract, whereas tumor cell apoptosis was increased compared to pair-fed controls. Microarray analysis of tumors identified significant changes in gene expression in the mushroom-fed mice as compared to controls. Gene network analysis identified alterations in networks involved in cell death, growth and proliferation, lipid metabolism, the TCA cycle and immune response. The data provided by this study illustrate the anticancer potential of phytochemicals in mushroom extract both in vitro and in vivo and supports the recommendation of white button mushroom as a dietary component that may aid in the prevention of prostate cancer in men.
白蘑菇是一种广泛食用的食物，含有对预防癌症有益的植物化学物质。本研究的目的是评估白蘑菇提取物及其主要成分共轭亚油酸（CLA）对前列腺癌细胞系的体外作用以及蘑菇提取物对体内的作用。在所有测试的细胞系中，蘑菇以剂量依赖的方式抑制细胞增殖，并在处理72小时内诱导细胞凋亡。CLA在体外抑制前列腺癌细胞系的增殖。用蘑菇提取物处理的裸鼠中，DU145和PC3前列腺肿瘤大小及肿瘤细胞增殖减少，而与配对喂养的对照组相比，肿瘤细胞凋亡增加。对肿瘤的微阵列分析表明，与对照组相比，喂食蘑菇的小鼠基因表达有显著变化。基因网络分析确定了参与细胞死亡、生长和增殖、脂质代谢、三羧酸循环和免疫反应的网络发生了改变。本研究提供的数据说明了蘑菇提取物中植物化学物质在体外和体内的抗癌潜力，并支持将白蘑菇推荐为一种可能有助于预防男性前列腺癌的膳食成分。
