Meng Dahai, Qiang Shigao, Lou Liguang, Zhao Weimin
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P. R. China.
Planta Med. 2008 Nov;74(14):1741-4. doi: 10.1055/s-2008-1081356. Epub 2008 Oct 20.
Bioassay-guided fractionation of the crude extract of Elaeocarpus hainanensis (Elaeocarpaceae), using the proliferation of non-small cell lung cancer A549/ATCC and human hepatocellular carcinoma BEL-7402 cells as a monitor, led to the isolation of ten cucurbitane-type triterpenoids, including three new compounds (1-3) and seven known compounds (4-10). Their structures were determined on the basis of spectroscopic analyses, chemical methods, and comparison with spectroscopic data in literature. The two known compounds, cucurbitacins D (5) and I (10) were found to exhibit the strongest cytotoxicity against A549/ATCC and BEL-7402 cells in vitro with IC50 values of less than 1 microM.
以非小细胞肺癌A549/ATCC细胞和人肝癌BEL-7402细胞的增殖为监测指标，对海南杜英（杜英科）粗提物进行生物活性导向的分离，得到了10个葫芦烷型三萜类化合物，包括3个新化合物（1-3）和7个已知化合物（4-10）。通过光谱分析、化学方法以及与文献光谱数据比较确定了它们的结构。发现两个已知化合物，葫芦素D（5）和I（10）在体外对A549/ATCC和BEL-7402细胞表现出最强的细胞毒性，IC50值小于1微摩尔。
