Chemaly Marianne, Toquin Marie-Therese, Le Nôtre Yolene, Fravalo Philippe
Laboratoire d'Etude de Recherche Avicole et Porcine, Unité Hygiène et Qualité des Produits Avicole et Porcin, BP 53-22440 Ploufragan, France.
J Food Prot. 2008 Oct;71(10):1996-2000. doi: 10.4315/0362-028x-71.10.1996.
This study aimed to update and create a data set from laying hens and broilers regarding contamination by Listeria monocytogenes. Two hundred laying-hen flocks were sampled, with 88 flocks reared in cages and 112 reared on the floor. One hundred forty-five broiler flocks were sampled, with 85 conventional and 60 free-range flocks. A total of 774 and 725 samples were analyzed from laying hens and broilers, respectively. L. monocytogenes was detected in 31 of 200 flocks, yielding an estimated prevalence of 15.5% in laying-hen flocks. Among positive flocks, there appeared a significant (P = 0.004) difference between caged and floor-reared hens, with a higher detection in dust samples from floor-reared hens. In positive caged hen flocks, significant (P = 0.028) differences between dust and fecal samples appeared, with a higher detection in feces than in dust samples. In broiler flocks, L. monocytogenes was isolated in 46 of 145 flocks, yielding an estimated prevalence of 32% (28% in conventional flocks versus 37% in the free-range flocks). L. monocytogenes was isolated in samples taken from conventional flocks with a lower frequency than in free-range flocks (13 versus 18%, respectively). The serotyping of L. monocytogenes strains showed that the majority belonged to type 1/2a in laying-hen flocks (74.3%) and in broiler flocks (40.5%). A significant difference (P = 0.007) between laying hens and broilers was shown for serogroup 4 and for serovar 1/2b (P = 0.007); these serogroups were more prevalent in broilers (40%) than in laying hens (5.7%).
本研究旨在更新并创建一个关于产蛋鸡和肉鸡中单核细胞增生李斯特菌污染情况的数据集。对200个产蛋鸡群进行了采样，其中88个鸡群采用笼养，112个鸡群采用地面平养。对145个肉鸡群进行了采样，其中85个为传统养殖鸡群，60个为散养鸡群。分别对774个产蛋鸡样本和725个肉鸡样本进行了分析。在200个产蛋鸡群中，有31个检测到单核细胞增生李斯特菌，估计产蛋鸡群中的患病率为15.5%。在阳性鸡群中，笼养母鸡和地面平养母鸡之间存在显著差异（P = 0.004），地面平养母鸡的灰尘样本中检测率更高。在阳性笼养产蛋鸡群中，灰尘样本和粪便样本之间存在显著差异（P = 0.028），粪便中的检测率高于灰尘样本。在肉鸡群中，145个鸡群中有46个分离出单核细胞增生李斯特菌，估计患病率为32%（传统养殖鸡群为28%，散养鸡群为37%）。从传统养殖鸡群采集的样本中分离出单核细胞增生李斯特菌的频率低于散养鸡群（分别为13%和18%）。单核细胞增生李斯特菌菌株的血清分型显示，在产蛋鸡群（74.3%）和肉鸡群（40.5%）中，大多数属于1/2a型。产蛋鸡和肉鸡在血清群4和血清型1/2b方面存在显著差异（P = 0.007）；这些血清群在肉鸡中（40%）比在产蛋鸡中（5.7%）更为普遍。
