Phytopathology. 2004 Feb;94(2):171-6. doi: 10.1094/PHYTO.2004.94.2.171.
ABSTRACT Effective biocontrol strains of Trichoderma virens can induce the production of defense-related compounds in the roots of cotton. Ineffective strains do not induce these compounds to significant levels. This elicittation was found to be heat stable, insoluble in chloroform, passed through a 5K molecular weight cut-off (MWCO) filter, but not a 3K MWCO filter, and was sensitive to treatment by proteinase K. When the active material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, several bands were present in the material from biocontrol-active strains that were lacking in inactive strains. When eluted and tested for elicitation activity, with or without renaturation, four bands stimulated cotton terpenoid production. One band showed cross-reaction with an antibody to the ethylene-inducing xylanase from T. viride. Another band of approximately 18 kDa, gave significant stimulation of cotton terpenoid production and increased peroxidase activity in cotton radicles in all tests, with or without renaturation. The 18-kDa protein was subjected to amino-terminal sequence analysis, and the first 19 amino acids at the amino terminus were determined to be DTVSYDTGYDNGSRSLNDV. A database homology search using the BLASTp algorithm showed the highest similarity to a serine proteinase from Fusarium sporotrichioides.
摘要 有效的生防菌层出镰刀菌能够诱导棉花根部产生防御相关化合物。无效菌株则不会诱导这些化合物产生显著水平的变化。这种诱导作用具有热稳定性，不溶于氯仿，可通过 5K 分子量截止（MWCO）过滤器，但不能通过 3K MWCO 过滤器，并且对蛋白酶 K 的处理敏感。当活性物质进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳时，生物防治活性菌株中的材料存在几种在非活性菌株中缺失的带。当洗脱并测试诱导活性时，无论是否复性，有四个带可刺激棉花萜烯的产生。一个带与来自绿木霉的乙烯诱导木聚糖酶的抗体发生交叉反应。另一个约 18 kDa 的带在所有测试中均能显著刺激棉花萜烯的产生并增加棉花胚根中的过氧化物酶活性，无论是否复性。该 18 kDa 蛋白进行了氨基末端序列分析，氨基末端的前 19 个氨基酸被确定为 DTVSYDTGYDNGSRSLNDV。使用 BLASTp 算法进行数据库同源性搜索显示，它与来自茄病镰刀菌的丝氨酸蛋白酶具有最高的相似性。
