Yu Wang, Wei Xiaowei, Zeng Qingpan, Lin Xiaoru, Zhou Xiaofu, Xu Hongwei
Jilin Provincial Key Laboratory of Plant Resources Science and Green Production, Jilin Normal University, Siping, 136000, People's Republic of China.
Sci Rep. 2025 Jul 22;15(1):26674. doi: 10.1038/s41598-025-12601-7.
In order to investigate the effects of Acer truncatum endophytic fungi Chaetomium globosum and Trichoderma asperellum on root configuration and growth of cucumber seedlings, "Xinjin No. 4" was used as experimental material and pot experiment was conducted. The experiment was set up with four treatments: a blank control (CK), a treatment with Chaetomium globosum at 100% concentration (CG), a treatment with Trichoderma asperellum at 100% concentration (TA), and a mixed strain treatment combining 50% Chaetomium globosum and 50% Trichoderma asperellum administered via root irrigation (GA). The effects of growth, root morphology, topological structure and typing parameters on root growth and development of Cucumis sativus L. (Xinjin No. 4) were studied. The results showed that, compared with the control group, the total root length, root surface area, root volume, cross number, plant height, stem diameter and biomass of cucumber seedlings treated with fungi solution were significantly increased, and the mixed fungi solution had the most significant effect on cucumber seedlings. The root system treated with fungi solution had obvious branching characteristics, such as forked branch structure, good fractal characteristics, small fractal dimension and high fractal abundance, indicating that its branch structure was relatively simple and had good expansion space, which was beneficial to occupying nutrient space. Soil water content, soil bulk density, soil porosity, soil capillary porosity and soil ventilation were the highest after the application of mixed fungi. The dominant particle size of aggregates of soil water stability under different treatments was 0.5-1 mm. The soil water stability, i.e. WR (the content of water-stable aggregates larger than 0.25 mm), mean weight diameter (MWD) and geometric mean diameter (GMD) values, were significantly increased after treatment with mixed fungi compared with the control group (p < 0.05), and the soil structure was more stable. In conclusion, single application of Chaetomium globosum or Trichoderma asperellum could significantly promote the growth of cucumber seedlings, but the effect of mixed solution was better than that of single solution, offering potential applications for sustainable agricultural practices to enhance crop productivity and soil health.
为了研究元宝枫内生真菌球毛壳菌和棘孢木霉对黄瓜幼苗根系构型及生长的影响，以“津研四号”为试验材料进行盆栽试验。试验设置4个处理：空白对照（CK）、100%浓度球毛壳菌处理（CG）、100%浓度棘孢木霉处理（TA）、50%球毛壳菌与50%棘孢木霉混合菌株通过灌根施用的处理（GA）。研究了生长、根系形态、拓扑结构及分型参数对黄瓜（津研四号）根系生长发育的影响。结果表明，与对照组相比，用真菌溶液处理的黄瓜幼苗的总根长、根表面积、根体积、交叉数、株高、茎粗和生物量均显著增加，且混合真菌溶液对黄瓜幼苗的影响最为显著。用真菌溶液处理的根系具有明显的分支特征，如叉状分支结构、良好的分形特征、较小的分形维数和较高的分形丰度，表明其分支结构相对简单且具有良好的扩展空间，有利于占据养分空间。施用混合真菌后，土壤含水量、土壤容重、土壤孔隙度、土壤毛管孔隙度和土壤通气性最高。不同处理下土壤水稳定性团聚体的优势粒径为0.5 - 1毫米。与对照组相比，混合真菌处理后土壤水稳定性即WR（大于0.25毫米水稳性团聚体含量）、平均重量直径（MWD）和几何平均直径（GMD）值显著增加（p < 0.05），土壤结构更稳定。综上所述，单独施用球毛壳菌或棘孢木霉均可显著促进黄瓜幼苗生长，但混合溶液的效果优于单一溶液，为可持续农业实践提高作物生产力和土壤健康提供了潜在应用。
