Phytopathology. 2002 Nov;92(11):1182-8. doi: 10.1094/PHYTO.2002.92.11.1182.
ABSTRACT To elucidate genetic mechanisms of the species-specific parasitism of Magnaporthe grisea, a Triticum isolate (pathogenic on wheat) was crossed with an Avena isolate (pathogenic on oat), and resulting F(1) progeny were subjected to segregation analyses on wheat cvs. Norin 4 and Chinese Spring. We found two fungal loci, Pwt3 and Pwt4, which are involved in the specific parasitism on wheat. Pwt3 operated on both cultivars while Pwt4 operated only on 'Norin 4'. Using the cultivar specificity of Pwt4, its corresponding resistance gene was successfully identified in 'Norin 4' and designated as Rmg1 (Rwt4). The presence of the corresponding resistance gene indicated that Pwt4 is an avirulence locus. Pwt3 was assumed to be an avirulence locus because of its temperature sensitivity. We suggest that gene-for-gene interactions underlie the species-specific parasitism of M. grisea.
摘要 为了阐明稻瘟病菌物种特异性寄生的遗传机制，我们将一个对小麦致病的小麦分离株与一个对燕麦致病的燕麦分离株进行杂交，然后对 F1 后代在小麦品种诺林 4 号和春小麦上进行分离分析。我们发现了两个与小麦特异性寄生有关的真菌基因座 Pwt3 和 Pwt4。Pwt3 在两个品种上均起作用，而 Pwt4 仅在“诺林 4 号”上起作用。利用 Pwt4 的品种特异性，我们在“诺林 4 号”中成功鉴定了其相应的抗性基因，并将其命名为 Rmg1（Rwt4）。相应抗性基因的存在表明 Pwt4 是一个无毒基因座。由于 Pwt3 的温度敏感性，我们假设它是一个无毒基因座。我们认为基因对基因相互作用是稻瘟病菌物种特异性寄生的基础。
