Phytopathology. 2000 Oct;90(10):1060-7. doi: 10.1094/PHYTO.2000.90.10.1060.
ABSTRACT A genetic cross was performed between a Setaria isolate (pathogenic on foxtail millet) and a Triticum isolate (pathogenic on wheat) of Magnaporthe grisea to elucidate genetic mechanisms of its specific parasitism toward wheat. A total of 80 F(1) progenies were obtained from 10 mature asci containing 8 ascospores. Lesions on wheat leaves produced by the F(1) progenies were classified into four types, which segregated in a 1:1:1:1 ratio. This result suggested that the pathogenicity of the F(1) population on wheat was controlled by two genes located at different loci. This idea was supported by backcross analyses. We designated these loci as Pwt1 and Pwt2. Cytological analyses revealed that Pwt1 and Pwt2 were mainly associated with the hypersensitive reaction and papilla formation, respectively.
摘要 为了阐明其对小麦特异性寄生的遗传机制，我们在玉蜀黍赤霉（Setaria isolate）（对谷子致病）和小麦赤霉（Triticum isolate）（对小麦致病）之间进行了遗传杂交。从含有 8 个孢子的 10 个成熟子囊中获得了总共 80 个 F(1)后代。F(1)后代在小麦叶片上产生的病变分为四种类型，它们以 1:1:1:1 的比例分离。这一结果表明，F(1)群体对小麦的致病性受位于不同基因座的两个基因控制。这一观点得到了回交分析的支持。我们将这些基因座命名为 Pwt1 和 Pwt2。细胞学分析表明，Pwt1 和 Pwt2 主要与过敏性反应和乳突形成有关。
