Phytopathology. 2001 Feb;91(2):181-7. doi: 10.1094/PHYTO.2001.91.2.181.
ABSTRACT A potential antagonist, Bacillus amyloliquefaciens strain RC-2, against Colletotrichum dematium, mulberry anthracnose fungus, was obtained from healthy mulberry leaves by in vitro and in vivo screening techniques. Application of culture filtrate of RC-2 inhibited disease on mulberry leaves, indicating that suppression was due to antifungal compounds in the filtrate. Development of mulberry anthracnose on mulberry leaves was inhibited only when the culture filtrate was applied before fungal inoculation, and it was not inhibited by application after inoculation. These results suggest that the antifungal compounds in the filtrate exhibit a preventive effect on the disease. Peptone significantly increased production of the antifungal compounds. The culture filtrate of RC-2 also inhibited the growth of several other phytopathogenic fungi and bacteria, such as Rosellinia necatrix, Pyricularia oryzae, Agrobacterium tumefaciens, and Xanthomonas campestris pv. campestris, in vitro. From the culture filtrate of RC-2, seven kinds of antifungal compounds were isolated by high performance liquid chromatography analysis, and one of the compounds was determined as iturin A2, a cyclic peptide, by nuclear magnetic resonance and fast atom bombardment mass analysis.
摘要 通过体外和体内筛选技术，从健康桑树叶中获得了一种潜在的拮抗真菌——解淀粉芽孢杆菌 RC-2，其可拮抗桑炭疽病菌。RC-2 菌株培养液滤液可抑制桑树叶上的炭疽病，表明抑菌作用是由于滤液中的抗真菌化合物所致。只有在接种真菌前施用培养液滤液才能抑制桑树叶上炭疽病的发生，而接种后施用则不能抑制病害的发生。这些结果表明，滤液中的抗真菌化合物对该病害具有预防作用。蛋白胨可显著提高抗真菌化合物的产量。RC-2 的培养液滤液在体外还可抑制其他几种植物病原菌和细菌的生长，如核盘菌、稻瘟病菌、根癌土壤杆菌和野油菜黄单胞菌 pv. 芸薹。通过高效液相色谱分析，从 RC-2 的培养液滤液中分离出 7 种抗真菌化合物，其中一种化合物通过核磁共振和快原子轰击质谱分析被确定为环状肽伊枯草菌素 A2。
