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附生酵母和细菌作为柑橘类水果绿霉病和青霉病的候选生物防治剂

Epiphytic Yeasts and Bacteria as Candidate Biocontrol Agents of Green and Blue Molds of Citrus Fruits.

作者信息

Hammami Rania, Oueslati Maroua, Smiri Marwa, Nefzi Souhaila, Ruissi Mustapha, Comitini Francesca, Romanazzi Gianfranco, Cacciola Santa Olga, Sadfi Zouaoui Najla

机构信息

Laboratoire de Mycologie, Pathologies et Biomarqueurs (LR16ES05), Département de Biologie, Université de Tunis-El Manar, Tunis 2092, Tunisia.

Laboratoire de Biotechnologie Appliquée à l'Agriculture, INRA Tunisie, Université de Carthage, Ariana 2049, Tunisia.

出版信息

J Fungi (Basel). 2022 Aug 3;8(8):818. doi: 10.3390/jof8080818.

DOI:10.3390/jof8080818
PMID:36012806
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9410262/
Abstract

Overall, 180 yeasts and bacteria isolated from the peel of citrus fruits were screened for their in vitro antagonistic activity against and , causative agents of green and blue mold of citrus fruits, respectively. Two yeast and three bacterial isolates were selected for their inhibitory activity on mycelium growth. Based on the phylogenetic analysis of 16S rDNA and ITS rDNA sequences, the yeast isolates were identified as and while the bacterial isolates were identified as , and . All five selected isolates significantly reduced the incidence of decay incited by and on 'Valencia' orange and 'Eureka' lemon fruits. Moreover, they were effective in preventing natural infections of green and blue mold of fruits stored at 4 °C. Treatments with antagonistic yeasts and bacteria did not negatively affect the quality and shelf life of fruits. The antagonistic efficacy of the five isolates depended on multiple modes of action, including the ability to form biofilms and produce antifungal lipopeptides, lytic enzymes and volatile compounds. The selected isolates are promising as biocontrol agents of postharvest green and blue molds of citrus fruits.

摘要

总体而言，从柑橘类水果果皮中分离出180株酵母菌和细菌，分别检测它们对柑橘绿霉病和青霉病病原菌（分别为 和 ）的体外拮抗活性。根据对2株酵母菌和3株细菌分离株对菌丝体生长的抑制活性，挑选出这些菌株。基于16S rDNA和ITS rDNA序列的系统发育分析，酵母菌分离株被鉴定为 和 ，而细菌分离株被鉴定为 、 和 。所有5株挑选出的分离株均显著降低了“巴伦西亚”橙和“尤力克”柠檬果实上由 和 引起的腐烂发生率。此外，它们在预防4℃储存的水果发生绿霉病和青霉病自然感染方面效果显著。用拮抗酵母菌和细菌处理对水果的品质和货架期没有负面影响。这5株分离株的拮抗功效取决于多种作用方式，包括形成生物膜以及产生抗真菌脂肽、裂解酶和挥发性化合物的能力。所选分离株有望成为柑橘类水果采后绿霉病和青霉病的生物防治剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/08a165ec1483/jof-08-00818-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/7c89383aa3c1/jof-08-00818-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/046aa06ab8e2/jof-08-00818-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/b6b9ccc91073/jof-08-00818-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/cbe839c3b752/jof-08-00818-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/8b4cd2c64631/jof-08-00818-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/f75c4c7dfdd1/jof-08-00818-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/5bd7bbd42a55/jof-08-00818-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/f02c0e0f65ac/jof-08-00818-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/08a165ec1483/jof-08-00818-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/7c89383aa3c1/jof-08-00818-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/046aa06ab8e2/jof-08-00818-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/b6b9ccc91073/jof-08-00818-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/cbe839c3b752/jof-08-00818-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/8b4cd2c64631/jof-08-00818-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/f75c4c7dfdd1/jof-08-00818-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/5bd7bbd42a55/jof-08-00818-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/f02c0e0f65ac/jof-08-00818-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bfa/9410262/08a165ec1483/jof-08-00818-g009.jpg

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