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对DA12的特性进行表征，其对产毒真菌具有强大的抗真菌活性。

Characterization of DA12 Showing Potent Antifungal Activity against Mycotoxigenic Species.

作者信息

Lee Theresa, Park Dami, Kim Kihyun, Lim Seong Mi, Yu Nan Hee, Kim Sosoo, Kim Hwang-Yong, Jung Kyu Seok, Jang Ja Yeong, Park Jong-Chul, Ham Hyeonheui, Lee Soohyung, Hong Sung Kee, Kim Jin-Cheol

机构信息

Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju 55365, Korea.

Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea.

出版信息

Plant Pathol J. 2017 Oct;33(5):499-507. doi: 10.5423/PPJ.FT.06.2017.0126. Epub 2017 Oct 1.

DOI:10.5423/PPJ.FT.06.2017.0126
PMID:29018313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5624492/
Abstract

In an attempt to develop a biological control agent against mycotoxigenic species, we isolated strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic , , , and both and (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including , , , , , and . The butanol extract of the culture filtrate of DA12 highly inhibited the germination of macroconidia with inhibition rate 83% at a concentration of 31.3 μg/ml and 100% at a concentration of 250 μg/ml. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of diseases and mycotoxin contamination of crops.

摘要

为了开发一种针对产毒真菌的生物防治剂，我们从土壤中分离出菌株DA12，并探究了其抗菌活性。DA12对产毒的、、、以及和（玉米）的生长均有抑制作用。采用对峙培养法进一步筛选发现，菌株DA12对其他真菌病原体也有活性，包括、、、、、和。DA12培养滤液的丁醇提取物在浓度为31.3μg/ml时对大分生孢子的萌发有高度抑制作用，抑制率为83%，在浓度为250μg/ml时抑制率为100%。通过薄层色谱 - 生物自显影法将丁醇提取物中的抗真菌代谢产物鉴定为伊枯草菌素A。此外，DA12产生的挥发性有机化合物能够抑制多种植物病原真菌的菌丝生长。通过气相色谱 - 质谱联用（GC-MS）分析，这些挥发性化合物被鉴定为2-庚酮、5-甲基庚酮和6-甲基庚酮。这些结果表明，DA12的拮抗活性归因于伊枯草菌素A和挥发性庚酮，该菌株可作为生物防治剂用于减少作物病害的发生和霉菌毒素污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/d3bfadf6cc13/ppj-33-499f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/2a3c0ba3c74a/ppj-33-499f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/c8903fcaac22/ppj-33-499f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/5da2367ed5d9/ppj-33-499f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/d3bfadf6cc13/ppj-33-499f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/2a3c0ba3c74a/ppj-33-499f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/c8903fcaac22/ppj-33-499f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/5da2367ed5d9/ppj-33-499f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b82/5624492/d3bfadf6cc13/ppj-33-499f4.jpg

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