Phytopathology. 2006 Mar;96(3):274-9. doi: 10.1094/PHYTO-96-0274.
ABSTRACT The population structure of Pepino mosaic virus (PepMV), which has caused severe epidemics in tomato in Spain since 2000, was analyzed. Isolates were characterized by the nucleotide sequence of the triple gene block and coat protein gene and, for a subset of isolates, a part of the RNA-dependent RNA polymerase gene. The full-length sequence of the genomic RNA of a Solanum muricatum isolate from Peru also was determined. In spite of high symptom diversity, the Spanish population of PepMV mostly comprised highly similar isolates belonging to the strain reported in Europe (European tomato strain), which has been the most prevalent genotype in Spain. The Spanish PepMV population was not structured spatially or temporally. Also, isolates highly similar to those from nontomato hosts from Peru (Peruvian strain) or to isolate US2 from the United States (US2 strain) were detected at lower frequency relative to the European strain. These two strains were detected in peninsular Spain only in 2004, but the Peruvian strain has been detected in the Canary Islands since 2000. These results suggest that PepMV was introduced into Spain more than once. Isolates from the Peruvian and US2 strains always were found in mixed infections with the European tomato strain, and interstrain recombinants were detected. The presence of different strains of the virus, and of recombinant isolates, should be considered for the development of control strategies based on genetic resistance.
摘要 自 2000 年以来，Pepino 花叶病毒（PepMV）已在西班牙的番茄上引发了严重的疫情，对其种群结构进行了分析。通过三基因块和外壳蛋白基因的核苷酸序列对分离株进行了特征描述，对于部分分离株，还对 RNA 依赖性 RNA 聚合酶基因的一部分进行了特征描述。此外，还确定了来自秘鲁的 Solanum muricatum 分离株的基因组 RNA 的全长序列。尽管症状多样性很高，但西班牙 PepMV 种群主要由高度相似的分离株组成，这些分离株属于在欧洲报道的毒株（欧洲番茄株系），这是西班牙最流行的基因型。西班牙 PepMV 种群在空间或时间上没有结构。此外，与来自秘鲁非番茄宿主（秘鲁株系）或来自美国的 US2 分离株（US2 株系）高度相似的分离株的检测频率相对较低，低于欧洲株系。这两种菌株仅在 2004 年在半岛西班牙被检测到，但秘鲁株系自 2000 年以来就在加那利群岛被检测到。这些结果表明 PepMV 不止一次被引入西班牙。秘鲁株系和 US2 株系的分离株总是与欧洲番茄株系混合感染，并且检测到了株间重组体。应考虑不同株系的病毒以及重组分离株的存在，以制定基于遗传抗性的控制策略。
