波兰新分离的番木瓜花叶病毒株与欧洲番茄、秘鲁和美国2株系有很大差异。

Pospieszny H, Borodynko N

Institute of Plant Protection, 60-318 Poznań, Poland.

Plant Dis. 2006 Aug;90(8):1106. doi: 10.1094/PD-90-1106C.

Pepino mosaic virus (PepMV, genus Potexvirus) was first described on pepino (Solanum muricatum) in Peru during 1980. Since 1999, the virus was reported in several European countries and in North and South America as an agent of viral disease of tomato crops. In Poland in 2002, the PepMV-SW isolate that was genetically similar to European isolates (approximately 99% identity) was identified (3). In November 2005, in the western part of the Wielkopolska Region, a virus with flexuous filamentous particles approximately 500 nm long was isolated from tomato fruits exhibiting symptoms of discoloration. Crude sap from Nicotiana benthamiana leaves was used for mechanical inoculation of indicator plants. The virus caused symptoms on N. benthamiana, N. clevelandii, Datura inoxia, and Lycopersicon esculentum. Symptomless infection on N. tabacum cv. Xanthi nc, N. tabacum cv. White Burley, and N. debneyi was confirmed by back-inoculation on N. benthamiana. The virus did not infect N. glutinosa, Physalis floridana, Petunia hybrida, Capsicum annuum, Chenopodium quinoa, Cucumis sativus, or Phaseolus vulgaris. The virus was initially identified using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antiserum against PepMV (DSMZ, Braunschweig, Germany). Positive serological reactions were obtained with sap from inoculated N. benthamiana, L. esculentum, and N. clevelandii plants. The serological identification was confirmed using a reverse transcription-polymerase chain reaction (RT-PCR) with primers generated from a sequence of the RNA polymerase region of an isolate of PepMV reported in the United Kingdom (1). Sequence information obtained from the amplified fragment of the virus designated PepMV-PK (GenBank Accession No. DQ387870), showed only 81% nt identity and 89% amino acid identity with PepMV-SW (GenBank Accession No. DQ387869). PepMV isolates can be divided into three strains including European tomato, Peruvian, and US2 based on their genetic diversity (2). The PepMV-PK isolate resulted in nucleotide identities ranging from 79 to 81% with isolates of the European tomato strain (GenBank Accession Nos. AJ438767, AF340024, AF484251, AJ271991, AJ606359, and AJ290424), 81% with the Peruvian strain (GenBank Accession Nos. AM109896 and AJ606361), and 78% identity with each of the U.S. isolates US1 (GenBank Accession No. AY509926) and US2 (GenBank Accession No. AY509927). These results show that the new Polish isolate is distinct from all other PepMV isolates reported to date. References: (1) C. J. French et al. Plant Dis. 85:1121, 2001. (2) L. Pagan et al. Phytopathology 96:274, 2006. (3) H. Pospieszny et al. Phytopathol. Pol. 26:91, 2002.

番木瓜环斑病毒（PepMV，马铃薯X病毒属）于1980年在秘鲁首次在番木瓜（Solanum muricatum）上被发现。自1999年以来，该病毒在几个欧洲国家以及北美洲和南美洲被报道为番茄作物的病毒病病原体。2002年在波兰，鉴定出了与欧洲分离株基因相似（约99%同源性）的PepMV-SW分离株（3）。2005年11月，在大波兰地区西部，从表现出变色症状的番茄果实中分离出一种具有约500纳米长的弯曲丝状颗粒的病毒。来自本氏烟草叶片的粗汁液被用于对指示植物进行机械接种。该病毒在本氏烟草、克利夫兰烟草、无刺曼陀罗和番茄上引起症状。通过将烟草品种Xanthi nc、烟草品种White Burley和德布尼烟草回接到本氏烟草上，证实了它们无症状感染。该病毒不感染黏毛烟草、弗罗里达酸浆、矮牵牛、辣椒、藜麦、黄瓜或菜豆。最初使用针对PepMV的多克隆抗血清通过双抗体夹心酶联免疫吸附测定（DAS-ELISA）鉴定该病毒（德国不伦瑞克的德国微生物和细胞培养物保藏中心）。对接种的本氏烟草、番茄和克利夫兰烟草植株的汁液获得了阳性血清学反应。使用从英国报道的PepMV分离株的RNA聚合酶区域序列生成的引物通过逆转录聚合酶链反应（RT-PCR）证实了血清学鉴定（1）。从命名为PepMV-PK的病毒扩增片段获得的序列信息（GenBank登录号DQ387870）显示，与PepMV-SW（GenBank登录号DQ387869）仅具有81%的核苷酸同源性和89%的氨基酸同源性。基于遗传多样性，PepMV分离株可分为三个株系，包括欧洲番茄株系、秘鲁株系和US2株系（2）。PepMV-PK分离株与欧洲番茄株系分离株（GenBank登录号AJ438767、AF340024、AF484251、AJ271991、AJ606359和AJ290424）的核苷酸同源性范围为79%至81%，与秘鲁株系（GenBank登录号AM109896和AJ606361）为81%，与美国分离株US1（GenBank登录号AY509926）和US2（GenBank登录号AY509927）各为78%同源性。这些结果表明，新的波兰分离株与迄今报道的所有其他PepMV分离株不同。参考文献：（1）C. J. French等人，《植物病害》85:1121，2001年。（2）L. Pagan等人，《植物病理学》96:274，2006年。（3）H. Pospieszny等人，《波兰植物病理学》26:91，2002年。