Schuitmaker J J, van Best J A, van Delft J L, Dubbelman T M, Oosterhuis J A, de Wolff-Rouendaal D
Department of Ophthalmology, State University of Leiden, The Netherlands.
Invest Ophthalmol Vis Sci. 1990 Aug;31(8):1444-50.
The photosensitizing properties of bacteriochlorin a (BCA), a nontoxic derivative of bacteriochlorophyll a, were investigated in vivo. BCA has an absorption band at a wavelength at which tissue penetration is optimal (760 nm), and it shows preferential tumor retention in Greene melanoma implanted in the anterior chamber of rabbit eyes. A dose of 20 mg/kg BCA was administered IV at 4-7 mm tumor diameter; 24 hr later the tumor was irradiated with near-infrared light (30 min, 760 nm, 150-280 J/cm2). On the day after the irradiation it appeared that tumor growth had stopped: fluorescein angiography showed nonperfusion of the tumor. Histopathology after enucleation showed subtotal tumor necrosis with occasionally small clusters of viable cells around a blood vessel and at the tumor periphery. Neither BCA nor light alone had any effect on the eye or melanoma.
研究了细菌叶绿素a的无毒衍生物细菌二氢卟吩a（BCA）在体内的光敏特性。BCA在组织穿透最佳的波长（760nm）处有一个吸收带，并且在植入兔眼前房的格林氏黑色素瘤中显示出优先的肿瘤滞留。在肿瘤直径为4 - 7mm时静脉注射20mg/kg的BCA；24小时后，用近红外光（30分钟，760nm，150 - 280J/cm²）照射肿瘤。照射后的第二天，肿瘤生长似乎停止：荧光素血管造影显示肿瘤无灌注。摘除眼球后的组织病理学显示肿瘤大部分坏死，偶尔在血管周围和肿瘤周边有小簇存活细胞。单独的BCA或光对眼睛或黑色素瘤均无任何影响。
