Phytopathology. 1999 May;89(5):374-9. doi: 10.1094/PHYTO.1999.89.5.374.
ABSTRACT Virus isolates from forage legumes collected from eight different states were identified as luteoviruses closely related to soybean dwarf luteovirus dwarfing (SbDV-D) and yellowing (SbDV-Y) described in Japan. All isolates produced reddened leaf margins in subterranean clover and were transmitted in a persistent manner by Acrythosiphon pisum, but not by Aulacorthum solani. Specific monoclonal antibodies raised against SbDV-Y were differentially reactive with endemic isolates. Immunoblots probed with a SbDV-D polyclonal antiserum showed single 26-kDa coat protein bands, confirming close serological relatedness to SbDV. Analyses of genomic and subgenomic double-stranded RNAs and northern blot analyses confirmed genomic relatedness to SbDV. Based on our results, we conclude that the U.S. luteovirus isolates studied comprise a strain or strains of the soybean dwarf virus that have clovers as common hosts and the pea aphid as a common vector.
摘要 从八个不同州采集的饲料豆科植物病毒分离物被鉴定为与在日本描述的大豆矮化花叶病毒矮化（SbDV-D）和黄化（SbDV-Y）密切相关的 luteoviruses。所有分离物都会导致三叶草的叶片边缘变红，并且可以通过豌豆蚜以持久性方式传播，但不能通过茄二十八星瓢虫传播。针对 SbDV-Y 产生的特异性单克隆抗体与地方性分离物具有不同的反应性。用 SbDV-D 多克隆抗血清探测免疫印迹显示出单一的 26kDa 外壳蛋白带，这证实了与 SbDV 的密切血清学相关性。对基因组和亚基因组双链 RNA 的分析以及 northern blot 分析证实了与 SbDV 的基因组相关性。根据我们的结果，我们得出结论，研究中的美国 luteovirus 分离物包含了一种或多种大豆矮化病毒株，这些病毒株以三叶草为共同宿主，以豌豆蚜为共同载体。
