Phytopathology. 1998 Nov;88(11):1192-9. doi: 10.1094/PHYTO.1998.88.11.1192.
ABSTRACT Infection of tobacco protoplasts or leaf tissues with peanut stunt virus (PSV) subgroup II strains induced the production of unusual cytoplasmic ribbon-like inclusions. The inclusion structures appeared as long, thin, densely staining sheets that were prevalent within the cytoplasm, accumulating most commonly near vacuoles. Numerous virions and ribosomes could be seen adjacent to the inclusion surfaces. The formation of these novel inclusions appeared to be subgroup specific, since infection of tobacco with PSV strains W and B (subgroup II), but not strains ER, V, and J (subgroup I), induced the inclusions. Furthermore, inclusion formation was shown to be host specific, because the inclusions were not detected in either of two leguminous host species infected with PSV subgroup II strains. Using tobacco protoplasts electroporated with various assortments of infectious RNA transcripts derived from cDNA clones of genomic RNAs of PSV-ER and PSV-W, we demonstrated that induction of the unusual ribbon-like inclusions maps to PSV-W (subgroup II) RNA3. This conclusion is consistent with the finding that PSV strain BV-15, a natural intraspecific reassortant that derives its RNA2 and RNA3 from a subgroup I strain, did not induce inclusion formation.
摘要 用花生丛矮病毒（PSV）亚组 II 株系感染烟草原生质体或叶片组织会诱导产生异常的细胞质带状内含物。这些内含物结构呈现出长而细、染色密集的薄片，在细胞质中普遍存在，最常见于液泡附近积累。可以看到大量病毒粒子和核糖体紧邻内含物表面。这些新型内含物的形成似乎具有亚组特异性，因为用 PSV 株系 W 和 B（亚组 II）而不是 ER、V 和 J（亚组 I）感染烟草会诱导内含物的形成。此外，内含物的形成具有宿主特异性，因为在感染 PSV 亚组 II 株系的两种豆科宿主物种中均未检测到内含物。使用来自 PSV-ER 和 PSV-W 基因组 RNA cDNA 克隆的各种传染性 RNA 转录本电穿孔烟草原生质体，我们证明了这种异常带状内含物的诱导与 PSV-W（亚组 II）RNA3 有关。这一结论与 PSV 株系 BV-15 的发现一致，BV-15 是一种天然的种内重组体，其 RNA2 和 RNA3 来自亚组 I 株系，不会诱导内含物的形成。
