Zeyong Xu, Higgins Colleen M, Kunrong Chen, Dietzgen Ralf G, Zhongyi Zhang, Liying Yang, Xiaoping Fang
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062 Wuhan, China.
QDPI Queensland Agricultural Biotechnology Centre, The University of Queensland, Qld 4072, Brisbane, Australia.
Plant Dis. 1998 Sep;82(9):992-998. doi: 10.1094/PDIS.1998.82.9.992.
On the basis of host reactions and serology, six Chinese peanut stunt virus (PSV) strains were found to be distinct from PSV-E and PSV-W, two type strains representing distinct serological subgroups. Chinese PSV strains were characterized by infecting Chenopodium amaranticolor and C. quinoa systemically. All Chinese strains were serologically closely related to each other, but distinct from PSV-E and more distant from PSV-W. Using two PSV-specific primers designed from conserved regions of the PSV RNA3 nucleotide sequence, cDNA transcribed from RNA3 of two Chinese PSV strains, Mi and S, was amplified by PCR and cloned. The sequenced cDNA of the two PSV strains included 654 nt of the coat protein (CP) gene. The identity of the CP gene nucleotide sequence between PSV-Mi and PSV-S was 99.0%, with 99.5% amino acid identity. Identity of the CP gene nucleotide sequence was 75.6 to 77.8% between PSV-Mi and -S (the two Chinese strains) and PSV-ER and -J in PSV subgroup I; and 74.1 to 74.4% between PSV-Mi and -S, and PSV-W in subgroup II. Based on these results, we propose placing PSV Chinese strains into a new PSV subgroup III.
基于寄主反应和血清学研究，发现6个中国花生矮化病毒（PSV）株系不同于PSV-E和PSV-W这两个代表不同血清学亚组的典型株系。中国PSV株系的特征是能系统侵染苋色藜和昆诺藜。所有中国株系在血清学上彼此密切相关，但不同于PSV-E，且与PSV-W的关系更远。利用根据PSV RNA3核苷酸序列保守区设计的2条PSV特异性引物，通过PCR扩增并克隆了两个中国PSV株系Mi和S的RNA3转录的cDNA。两个PSV株系的测序cDNA包含外壳蛋白（CP）基因的654个核苷酸。PSV-Mi和PSV-S之间CP基因核苷酸序列的同一性为99.0%，氨基酸同一性为99.5%。PSV-Mi和-S（两个中国株系）与PSV亚组I中的PSV-ER和-J之间CP基因核苷酸序列的同一性为75.6%至77.8%；PSV-Mi和-S与亚组II中的PSV-W之间的同一性为74.1%至74.4%。基于这些结果，我们建议将中国PSV株系归入一个新的PSV亚组III。
