Phytopathology. 1998 May;88(5):410-5. doi: 10.1094/PHYTO.1998.88.5.410.
ABSTRACT Molecular markers linked to quantitative trait loci conditioning tolerance to barley yellow dwarf virus (BYDV) were identified in oat (Avena sativa) using amplified fragment length polymorphism (AFLP) analysis. Near-isogenic and recombinant inbred lines (NILs and RILs, respectively) derived from a cross of Clintland64 (BYDV-sensitive) and IL86-5698 (BYDV-tolerant) were evaluated for their responses to an Illinois isolate of the PAV strain of BYDV. Individual markers identified in the analysis of the NILs explained up to 35% of the variability seen in the tolerance response. Single-point analysis of the marker data from the RIL population identified 24 markers in three linkage groups that were associated with tolerance to BYDV infection at P </= 0.001. These markers defined three major loci, A, C, and E, that were contributed by the tolerant parent (IL86-5698) and explained 35.0, 20.6, and 17.0% of the variability, respectively. Three minor loci G, H(1), and R) were identified at P </= 0.01. These loci were contributed by the sensitive parent (Clintland64) and explained 5.8, 5.6, and 5.6% of the variability, respectively. Interval analysis showed that only the loci A, C, and E are associated significantly with BYDV tolerance at log of the likelihood ratio >/= 3.0. These loci explained about 50% total of the variation in BYDV tolerance in multimarker regression analysis in both years. The BYDV tolerance loci A, C, E, and R were mapped to hexaploid oat restriction fragment length polymorphism linkage groups 2, 8, 36, and 5, respectively, by analyzing the segregation of the AFLP markers in the Kanota x Ogle RIL population.
摘要 利用扩增片段长度多态性 (AFLP) 分析，在燕麦 (Avena sativa) 中鉴定出与大麦黄花叶病毒 (BYDV) 数量性状位点相关的分子标记。近等基因系 (NILs) 和重组自交系 (RILs) 分别来自 Clintland64 (BYDV 敏感) 和 IL86-5698 (BYDV 耐受) 的杂交，用于评估它们对伊利诺伊州 PAV 株系 BYDV 的反应。在 NILs 分析中鉴定出的个别标记解释了对 BYDV 感染的耐受反应中观察到的 35%的可变性。对 RIL 群体标记数据的单点分析在三个连锁群中确定了 24 个标记，这些标记与 BYDV 感染的耐受性相关，在 P </= 0.001 时达到显著水平。这些标记定义了三个主要位点 A、C 和 E，它们由耐受亲本 (IL86-5698) 贡献，分别解释了 35.0%、20.6%和 17.0%的可变性。在 P </= 0.01 时还确定了三个次要位点 G、H(1) 和 R)。这些位点由敏感亲本 (Clintland64) 贡献，分别解释了 5.8%、5.6%和 5.6%的可变性。区间分析表明，只有 A、C 和 E 位点与 BYDV 耐受相关，对数似然比> = 3.0。这些位点在两年的多标记回归分析中解释了 BYDV 耐受总变异的约 50%。通过分析 Kanota x Ogle RIL 群体中 AFLP 标记的分离，将 BYDV 耐受位点 A、C、E 和 R 分别映射到六倍体燕麦限制性片段长度多态性连锁群 2、8、36 和 5。
