Phytopathology. 1998 Jul;88(7):673-7. doi: 10.1094/PHYTO.1998.88.7.673.
ABSTRACT Nine transformants of Trichoderma longibrachiatum with extra copies of the egl1 gene were studied for mitotic stability, endoglucanase production, and biocontrol activity against Pythium ultimum on cucumber seedlings. The transformants showed a significantly higher level of expression of the egl1 gene in comparison to the wild type under both inducing and noninducing growth conditions. Transformants with the egl1 gene under the control of a constitutive promoter had the highest enzymatic activity. Both the endoglucanase activity and the transforming sequences were stable under nonselective conditions. When applied to cucumber seeds sown in P. ultimum-infested soil, T. longibrachiatum transformants with increased inducible or constitutive egl1 expression generally were more suppressive than the wild-type strain.
研究了 9 株具有 egl1 基因额外拷贝的长枝木霉转化体，以评估其在有丝分裂稳定性、内切葡聚糖酶生产和对黄瓜幼苗上的终极腐霉的生物防治活性方面的表现。与野生型相比，在诱导和非诱导生长条件下，转化体的 egl1 基因表达水平显著提高。在组成型启动子控制下的 egl1 基因转化体具有最高的酶活性。在非选择性条件下，内切葡聚糖酶活性和转化序列均稳定。当将其应用于播种在终极腐霉侵染土壤中的黄瓜种子时，与野生型菌株相比，诱导型或组成型 egl1 表达增强的长枝木霉转化体通常具有更强的抑制作用。
