Hinterberger Valentin, Douchkov Dimitar, Lück Stefanie, Kale Sandip, Mascher Martin, Stein Nils, Reif Jochen C, Schulthess Albert W
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.
German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany.
Front Plant Sci. 2022 Mar 1;13:836723. doi: 10.3389/fpls.2022.836723. eCollection 2022.
Genetic pathogen control is an economical and sustainable alternative to the use of chemicals. In order to breed resistant varieties, information about potentially unused genetic resistance mechanisms is of high value. We phenotyped 8,316 genotypes of the winter wheat collection of the , for resistance to powdery mildew (PM), , one of the most important biotrophic pathogens in wheat. To achieve this, we used a semi-automatic phenotyping facility to perform high-throughput detached leaf assays. This data set, combined with genotyping-by-sequencing (GBS) marker data, was used to perform a genome-wide association study (GWAS). Alleles of significantly associated markers were compared with SNP profiles of 171 widely grown wheat varieties in Germany to identify currently unexploited resistance conferring genes. We also used the Chinese Spring reference genome annotation and various domain prediction algorithms to perform a domain enrichment analysis and produced a list of candidate genes for further investigation. We identified 51 significantly associated regions. In most of these, the susceptible allele was fixed in the tested commonly grown wheat varieties. Eleven of these were located on chromosomes for which no resistance conferring genes have been previously reported. In addition to enrichment of leucine-rich repeats (LRR), we saw enrichment of several domain types so far not reported as relevant to PM resistance, thus, indicating potentially novel candidate genes for the disease resistance research and prebreeding in wheat.
基因病原体控制是一种经济且可持续的化学药剂替代方法。为了培育抗性品种，有关潜在未利用的遗传抗性机制的信息具有很高的价值。我们对冬小麦种质库中的8316个基因型进行了表型分析，以检测其对白粉病（PM）的抗性，白粉病是小麦中最重要的活体营养型病原体之一。为此，我们使用了半自动表型分析设备进行高通量离体叶片测定。该数据集与简化基因组测序（GBS）标记数据相结合，用于进行全基因组关联研究（GWAS）。将显著关联标记的等位基因与德国171个广泛种植的小麦品种的单核苷酸多态性（SNP）图谱进行比较，以鉴定当前未被利用的抗性赋予基因。我们还利用中国春参考基因组注释和各种结构域预测算法进行结构域富集分析，并生成了一份候选基因清单以供进一步研究。我们鉴定出51个显著关联区域。在大多数这些区域中，感病等位基因在测试的常见种植小麦品种中是固定的。其中11个位于此前未报道有抗性赋予基因的染色体上。除了富含亮氨酸重复序列（LRR）的富集外，我们还发现了几种迄今未报道与白粉病抗性相关的结构域类型的富集，因此，这表明在小麦抗病研究和育种前阶段可能存在新的候选基因。
