Suwanpugdee A, Kornkeawrat K, Saikhun K, Siriaroonrat B, Tipkantha W, Doungsa-Ard K, Sa-Ardrit M, Suthunmapinatha P, Pinyopummin A
Center of Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 73140, Thailand.
Theriogenology. 2009 Mar 1;71(4):576-85. doi: 10.1016/j.theriogenology.2008.08.022. Epub 2008 Oct 21.
The serow (Capricornis sumatraensis) is a critically endangered species. The objectives of this study were to evaluate ejaculate quality in captive males, and to investigate and characterize sperm morphology. Semen was collected using electroejaculation. Mean (+/-S.D.) seminal characteristics were: semen volume 2.3+/-0.8 mL, pH 7.8+/-0.4, and osmolality 329.9+/-32.9mOsmol/kg; sperm concentration 515.8+/-263.1 x 10(6) cells/mL; wave motion score (1-5) 3.9+/-0.4; motile sperm 60.5+/-22%; viable sperm 68.3+/-9.4%; morphologically normal sperm 70.8+/-19.3%; and an opacity that was yellowish to milky-white. Sperm head length, width, degree of elongation, area, and perimeter were 6.0+/-0.6 microm, 4.3+/-0.3 microm, 71.7+/-8.6%, 19.8+/-2.5 microm(2), and 17.9+/-2.1 microm. Based on these measurements, we categorized sperm head morphometry as small, medium, or large. In addition, sperm morphology was examined by light and scanning electron microscopy; overall, morphologically normal and abnormal sperm were similar to those reported for other bovidae. In summary, this study provided baseline data regarding semen characteristics of C. sumatraensis, which should be of value in the preservation of this endangered species.
鬣羚（苏门羚）是一种极度濒危的物种。本研究的目的是评估圈养雄性鬣羚的射精质量，并调查和描述精子形态。采用电刺激采精法采集精液。精液的平均（±标准差）特征如下：精液体积2.3±0.8 mL，pH值7.8±0.4，渗透压329.9±32.9 mOsmol/kg；精子浓度515.8±263.1×10⁶个细胞/mL；活力评分（1 - 5分）3.9±0.4；活动精子60.5±22%；活精子68.3±9.4%；形态正常精子70.8±19.3%；精液呈淡黄色至乳白色。精子头部的长度、宽度、伸长率、面积和周长分别为6.0±0.6微米、4.3±0.3微米、71.7±8.6%、19.8±2.5平方微米和17.9±2.1微米。基于这些测量结果，我们将精子头部形态测量分为小、中、大三类。此外，通过光学显微镜和扫描电子显微镜检查精子形态；总体而言，形态正常和异常的精子与其他牛科动物报道的情况相似。总之，本研究提供了关于苏门羚精液特征的基线数据，这对于保护这种濒危物种具有重要价值。
