Keijser B J F, Zaura E, Huse S M, van der Vossen J M B M, Schuren F H J, Montijn R C, ten Cate J M, Crielaard W
TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Zeist, The Netherlands.
J Dent Res. 2008 Nov;87(11):1016-20. doi: 10.1177/154405910808701104.
A good definition of commensal microflora and an understanding of its relation to health are essential in preventing and combating disease. We hypothesized that the species richness of human oral microflora is underestimated. Saliva and supragingival plaque were sampled from 71 and 98 healthy adults, respectively. Amplicons from the V6 hypervariable region of the small-subunit ribosomal RNA gene were generated by PCR, pooled into saliva and plaque pools, and sequenced by means of the Genome Sequencer 20 system at 454 Life Sciences. Data were evaluated by taxonomic and rarefaction analyses. The 197,600 sequences generated yielded about 29,000 unique sequences, representing 22 taxonomic phyla. Grouping the sequences in operational taxonomic units (6%) yielded 3621 and 6888 species-level phylotypes in saliva and plaque, respectively. This work gives a radically new insight into the diversity of human oral microflora, which, with an estimated number of 19,000 phylotypes, is considerably higher than previously reported.
对共生微生物群的良好定义及其与健康关系的理解对于预防和抗击疾病至关重要。我们推测人类口腔微生物群的物种丰富度被低估了。分别从71名和98名健康成年人中采集唾液和龈上菌斑。通过PCR扩增小亚基核糖体RNA基因的V6高变区的扩增子，汇集到唾液和菌斑池中，并在454生命科学公司的基因组测序仪20系统上进行测序。通过分类学和稀疏分析对数据进行评估。生成的197,600个序列产生了约29,000个独特序列，代表22个分类门。将序列分组为操作分类单元（6%），在唾液和菌斑中分别产生了3621种和6888种水平的系统发育型。这项工作为人类口腔微生物群的多样性提供了全新的见解，其估计的系统发育型数量为19,000种，大大高于先前报道的数量。
