Wijnker J J, Depner K R, Berends B R
Institute for Risk Assessment Sciences, Division Veterinary Public Health, P.O. Box 80.175 NL-3508 TD, University of Utrecht, The Netherlands.
Int J Food Microbiol. 2008 Dec 10;128(2):411-3. doi: 10.1016/j.ijfoodmicro.2008.09.013. Epub 2008 Sep 30.
Pig intestines used for the production of natural sausage casings may carry classical swine fever (CSF) virus. Feeding pigs with human food waste that contains pig casings may then spread the virus to CSF-free animals. Casings derived from a pig experimentally infected with CSF by dosing with 10(6) tissue culture infectious doses (TCID50) of the highly virulent CSF virus strain "Koslov", were treated with phosphate supplemented or citrate supplemented NaCl, instead of with NaCl alone, which is the standard preservation treatment for casings. Treated casings were stored for 30 days at either 4 degrees C or 20 degrees C. After storage the casings were fed to 16 susceptible pigs. CSF infection was confirmed in the four animals that had been fed casings treated with citrate supplemented salt and stored at 4 degrees C. All other animals remained healthy. It is therefore possible to avoid the inadvertent spread of CSF virus via porcine sausage casings by treating casings with phosphate supplemented salt and storing them for 30 days at temperatures over 4 degrees C.
用于生产天然肠衣的猪肠可能携带经典猪瘟（CSF）病毒。用含有猪肠衣的人类食物残渣喂猪可能会将病毒传播给无CSF的动物。从通过用高毒力CSF病毒株“Koslov”的10(6)组织培养感染剂量（TCID50）给药而实验感染CSF的猪获得的肠衣，用补充了磷酸盐或柠檬酸盐的NaCl处理，而不是仅用NaCl处理，NaCl是肠衣的标准保存处理方法。处理后的肠衣在4℃或20℃下储存30天。储存后，将肠衣喂给16头易感猪。在用补充了柠檬酸盐的盐处理并在4℃下储存的肠衣喂养的4只动物中确认了CSF感染。所有其他动物保持健康。因此，通过用补充了磷酸盐的盐处理肠衣并在4℃以上的温度下储存30天，可以避免CSF病毒通过猪肠衣的意外传播。
