da Silva Paulo Tadeu, Pappen Fernanda Geraldes, Souza Erick Miranda, Dias João Eduardo, Bonetti Filho Idomeo, Carlos Iracilda Zepponi, Leonardo Renato Toledo
Department of Restorative Dentistry, Dental School of Araraquara, State University of São Paulo, Araraquara, SP, Brazil.
Braz Dent J. 2008;19(3):228-31. doi: 10.1590/s0103-64402008000300010.
This study evaluated in vitro the cytotoxicity of four root canal sealers (Topseal, EndoRez, TubliSeal and Kerr Pulp Canal Sealer E.W.T.) and their effects on reactive oxygen/nitrogen intermediate induction by mouse peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 mL 10 mM phosphate-buffered saline, washed twice and resuspended (10(6) cells/mL) in appropriate medium for each test. Cytotoxicity was determined by the presence of hydrogen peroxide (H2O2) and nitric oxide (NO) by the peroxidase-dependent oxidation of phenol red and Griess reaction, respectively. Sealer suspensions were obtained in two different concentrations from each material: 18 mg/mL and 9 mg/mL, established according to compatibility parameters following MTT assay. Comparing the sealers, H2O2 release at concentrations of 9 mg/mL and 18 mg/mL was similar: Topseal > positive control (medium + cells + 5 mg/mL zimozan solution) > EndoRez > TubliSeal > Kerr Pulp E.W.T. > negative control (medium + cells). NO release at concentration of 9 mg/mL was: positive control (medium + cells + 10 microg/mL LPS solution) > Topseal > Kerr Pulp E.W.T. > TubliSeal = EndoRez > negative control (medium + cells); at concentration of 18 mg/mL was: positive control > Topseal > Kerr Pulp E.W.T > TubliSeal > EndoRez > negative control. Based on the results, it may be concluded that Topseal presented the highest cytotoxicity among the tested sealers, releasing higher concentrations of NO and H2O2 in macrophage culture.
本研究在体外评估了四种根管封闭剂（Topseal、EndoRez、TubliSeal和Kerr牙髓根管封闭剂E.W.T.）的细胞毒性及其对小鼠腹腔巨噬细胞诱导产生活性氧/氮中间体的影响。通过用5 mL 10 mM磷酸盐缓冲盐水对瑞士小鼠进行腹腔灌洗获得经巯基乙酸盐诱导的细胞，洗涤两次后重悬于（1×10⁶细胞/mL）适合每次测试的培养基中。分别通过酚红的过氧化物酶依赖性氧化和格里斯反应，根据过氧化氢（H₂O₂）和一氧化氮（NO）的存在情况来测定细胞毒性。从每种材料获得两种不同浓度的封闭剂悬浮液：18 mg/mL和9 mg/mL，这是根据MTT试验后的相容性参数确定的。比较这些封闭剂，9 mg/mL和18 mg/mL浓度下H₂O₂的释放情况相似：Topseal＞阳性对照（培养基+细胞+5 mg/mL齐莫赞溶液）＞EndoRez＞TubliSeal＞Kerr牙髓E.W.T.＞阴性对照（培养基+细胞）。9 mg/mL浓度下NO的释放情况为：阳性对照（培养基+细胞+10 μg/mL LPS溶液）＞Topseal＞Kerr牙髓E.W.T.＞TubliSeal = EndoRez＞阴性对照（培养基+细胞）；18 mg/mL浓度下为：阳性对照＞Topseal＞Kerr牙髓E.W.T＞TubliSeal＞EndoRez＞阴性对照。根据结果可以得出结论，在测试的封闭剂中，Topseal具有最高的细胞毒性，在巨噬细胞培养中释放更高浓度的NO和H₂O₂。
