念珠藻属蓝细菌中乙酰胆碱酯酶抑制活性的筛选。

Screening for acetylcholinesterase inhibitory activity in cyanobacteria of the genus Nostoc.

作者信息

Zelík Petr, Lukesová Alena, Voloshko Ludmila N, Stys Dalibor, Kopecký Jirí

机构信息

Faculty of Chemistry, Institute of Food Chemistry and Biotechnology, Brno University of Technology, Brno, Czech Republic.

出版信息

J Enzyme Inhib Med Chem. 2009 Apr;24(2):531-6. doi: 10.1080/14756360802234836.

DOI:10.1080/14756360802234836

PMID:18951237

Abstract

Fifty-four cyanobacterial strains of the genus Nostoc from different habitats were screened for acetylcholinesterase inhibitory activity. Water-methanolic extracts from freeze-dried biomasses were tested for inhibitory activity using Ellman's spectrophotometric method. Acetylcholinesterase inhibitory activity higher than 90% was found in the crude extracts of Nostoc sp. str. Lukesova 27/97 and Nostoc ellipsosporum Rabenh. str. Lukesova 51/91. Extracts from Nostoc ellipsosporum str. Lukesova 52/91 and Nostoc linckia f. muscorum (Ag.) Elenk. str. Gromov, 1988, CALU-980 inhibited AChE activity by 84.9% and 65.3% respectively. Moderate AChE inhibitory activity (29.1-37.5%) was found in extracts of Nostoc linckia Roth. str. Gromov, 1962/10, CALU-129, Nostoc muscorum Ag. str. Lukesova 127/97, Nostoc sp. str. Lhotsky, CALU-327 and Nostoc sp. str. Gromov, CALU-998. Extracts from another seven strains showed weak anti-AChE activities. The active component responsible for acetylcholinesterase inhibition was identified in a crude extract of Nostoc sp. str. Lukesova 27/97 using HPLC and found to occur in one single peak.

摘要

对来自不同生境的54株念珠藻属蓝藻菌株进行了乙酰胆碱酯酶抑制活性筛选。使用埃尔曼分光光度法对冻干生物量的水 - 甲醇提取物进行抑制活性测试。在 Nostoc sp. str. Lukesova 27/97 和 Nostoc ellipsosporum Rabenh. str. Lukesova 51/91 的粗提物中发现乙酰胆碱酯酶抑制活性高于90%。Nostoc ellipsosporum str. Lukesova 52/91 和 Nostoc linckia f. muscorum (Ag.) Elenk. str. Gromov, 1988, CALU - 980 的提取物分别抑制乙酰胆碱酯酶活性84.9%和65.3%。在 Nostoc linckia Roth. str. Gromov, 1962/10, CALU - 129、Nostoc muscorum Ag. str. Lukesova 127/97、Nostoc sp. str. Lhotsky, CALU - 327 和 Nostoc sp. str. Gromov, CALU - 998 的提取物中发现了中等程度的乙酰胆碱酯酶抑制活性（29.1 - 37.5%）。另外七株菌株的提取物显示出较弱的抗乙酰胆碱酯酶活性。使用高效液相色谱法在 Nostoc sp. str. Lukesova 27/97 的粗提物中鉴定出负责抑制乙酰胆碱酯酶的活性成分，发现其出现在一个单一峰中。