Li Chang-Che, Yeh Su-Tuen, Chen Jiann-Chu
Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.
Fish Shellfish Immunol. 2008 Dec;25(6):853-60. doi: 10.1016/j.fsi.2008.09.014. Epub 2008 Oct 7.
White shrimp Litopenaeus vannamei injected with saline, and injected with tryptic soy broth (TSB)-grown Vibrio alginolyticus at 1.0 x 10(5) and 1.8 x 10(5) colony-forming units (cfu) shrimp(-1) were examined for hyaline cell (HC) counts, granular cell (GC) counts, total haemocyte counts (THCs), phenoloxidase (PO) activity, respiratory burst (RB) and superoxide dismutase (SOD) activity after 1-168 h. Shrimp that received no injection served as the control. The shrimps which received V. alginolyticus at both doses showed significant decreases in these parameters after 6-96 h. The values for HC and SOD activity decreased earlier and then RB. The time to cause maximum depletion of haemocytes (haemocytopenia), PO activity, RB, and SOD activity were 12, 72, 48, and 24 h post-injection, respectively. The HC, GC, and RB returned to the original values earlier at 72 h, followed by SOD activity at 96 h, and then PO activity at 168 h post-infection. It was concluded that an injection of V. alginolyticus rapidly reduced the shrimp's immunity by decreasing HC, GC, SOD activity, RB, and PO activity within 3-24 h, followed by a slow recovery during 72-168 h post-injection. Furthermore, white shrimp L. vannamei which received V. alginolyticus showed a 6-9 h later response in PO activity, and a 72-96 h later recovery of PO activity, compared to the responses in RB and SOD activity indicating their roles in shrimp defence and immunity.
将凡纳滨对虾注射生理盐水，并分别以每尾虾1.0×10⁵和1.8×10⁵菌落形成单位（cfu）注射胰蛋白胨大豆肉汤（TSB）培养的溶藻弧菌，在1 - 168小时后检测其透明细胞（HC）计数、颗粒细胞（GC）计数、总血细胞计数（THC）、酚氧化酶（PO）活性、呼吸爆发（RB）和超氧化物歧化酶（SOD）活性。未注射的对虾作为对照。接受两种剂量溶藻弧菌注射的对虾在6 - 96小时后这些参数均显著下降。HC和SOD活性的值下降较早，然后是RB。导致血细胞最大耗竭（血细胞减少）、PO活性、RB和SOD活性的时间分别为注射后12、72、48和24小时。HC、GC和RB在72小时较早恢复到原始值，其次是96小时的SOD活性，然后是感染后168小时的PO活性。得出的结论是，注射溶藻弧菌在3 - 24小时内通过降低HC、GC、SOD活性、RB和PO活性迅速降低对虾的免疫力，随后在注射后72 - 168小时缓慢恢复。此外，与RB和SOD活性的反应相比，接受溶藻弧菌注射的凡纳滨对虾在PO活性上有6 - 9小时的延迟反应，并且PO活性在72 - 96小时后恢复，表明它们在对虾防御和免疫中的作用。
